Planta Med
DOI: 10.1055/s-0043-115224
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Decontamination of Mikania glomerata Leaves by Gamma Irradiation: Coumarin Determination by HPLC-DAD, Microbiological Control and Genotoxicological Studies

Marcos Martins Gouvêa1, 2, 9, Samara Cristina Ferreira-Machado3, 4, Thalita Martins da Silva1, 2, Julien Silva Lima3, Cláudio Sérgio Corrêa Lau3, Luciana Maria Ramires Esper5, 6, Samanta Cardozo Mourão1, 2, 5, 7, Elizabeth Valverde Macedo1, 2, 7, Helio de Carvalho Vital8, Edson Ramos Andrade8, Annibal Duarte Pereira Netto9, 10, Flávia Ferreira de Carvalho Marques9, 10, Carlos Augusto de Freitas Peregrino1, 2, 7
  • 1Pró-Reitoria de Extensão, LURA, Universidade Federal Fluminense, Niterói, Brazil
  • 2Instituto Vital Brazil, LDNF, Niterói, Brazil
  • 3Instituto de Biologia Roberto Alcântara Gomes, LCR, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil
  • 4Departamento de Biologia Geral, Universidade Federal Fluminense, Niterói, Brazil
  • 5Programa de Pós-Graduação em Ciências Aplicadas a Produtos da Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, Brazil
  • 6Departamento de Bromatologia, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, Brazil
  • 7Departamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, Brazil
  • 8Centro Tecnológico do Exército, Rio de Janeiro, Brazil
  • 9Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal Fluminense, Niterói, Brazil
  • 10Departamento de Química Analítica, LaQAFA, Instituto de Química, Universidade Federal Fluminense, Niterói, Brazil
Further Information

Publication History

received 20 November 2016
revised 08 June 2017

accepted 22 June 2017

Publication Date:
12 July 2017 (eFirst)


Gamma irradiation as a decontaminating physical agent could be an important tool in the production chain of herbal medicines by improving the microbiological quality of raw materials and the safety of final products. This study was undertaken to investigate the genotoxic potential and eventual chemical modifications of a batch of Mikania glomerata raw material decontaminated by different doses of gamma irradiation (2.0, 3.5, and 5.0 kGy), using a cesium-137 source. DNA damage was assessed in vitro by agarose gel electrophoresis in regard to double-chain breaks of plasmid pUC 9.1 DNA and in vivo by micronucleus test in bone marrow cells of Wistar rats. Cytotoxicity in bone marrows was assessed by scoring polychromatic and normochromatic erythrocytes ratio. An HPLC-DAD method was adapted and validated for the enhancement of coumarin selectivity from the other matrix constituents. The microbial load was satisfactorily reduced, leading to sterilization at the highest dose. Genotoxic and cytotoxic effects were not increased in the in vitro and in vivo models. The concentration of coumarin and the chromatographic profiles of the hydroalcoholic plant extracts (ethanol 70% v/v) were not modified after such process. Therefore, this work suggests that gamma irradiation of M. glomerata raw material is suitable and safe for microbiological control purposes at the employed doses.

Supporting Information