Abstract
The ingredients of Actovegin® were analyzed and its effects on the muscle cell proliferation were investigated.
C2C12 myoblasts were cultured in medium. Actovegin® was added in five different concentrations (1, 5, 25, 125, and 250 µg) to the differentiation
medium. The formations of proliferation factor Ki67 and myosin heavy chains were measured
by immunofluorescence. The first primary antibody was anti-Ki67 and anti-Mf20. Cells
were washed and treated with the second fluorochrome. Thirty-one Actovegin® ingredients were found to contain significantly higher concentrations and twenty-nine
ingredients were found to contain significantly lower concentrations, compared to
the mean ranges as described in the literature for the normal physiological concentrations
in human adult serum/plasma. A significant increase in the formation of Ki67 was observed
in Actovegin® groups, compared to controls. The mean area of myotubes was significantly increased
in Actovegin® groups. A significant decrease in the number of myotubes was observed. An increased
myotube size (fusion) was observed. The intensity of Mf20 was significantly increased
in Actovegin® groups. It could be demonstrated that Actovegin® contains many physiological substances in significantly higher and some in lower
concentrations compared to human adult serum. Furthermore, it could be shown that
Actovegin® improves muscle cell proliferation.
Key word
Actovegin
®
- analysis - C2C12 myoblasts - proliferation - Ki67 - MF20
