Planta Med
DOI: 10.1055/s-0043-117414
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Rapid and Sensitive UPLC-MS/MS Method for Quantification of Bruceantinol in Rat Plasma and Its Application to a Pharmacokinetic Study

Jun Li1, 2, Dayong Zheng3, Bixian Zhen2, Yunfeng Sun1, Fen Chen2, Kaixuan Zhan2, Ning Wei4, Lide Zhang2
  • 1Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, China
  • 2Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China
  • 3Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, China
  • 4Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, USA
Further Information

Publication History

received 13 April 2017
revised 05 July 2017

accepted 20 July 2017

Publication Date:
31 July 2017 (eFirst)

Abstract

Bruceantinol (BOL), a quassinoid compound isolated from the fruits of Brucea javanica, has been reported to have cytotoxic and antibacterial effects. In this study, a rapid, sensitive, and specific ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed for the quantitative determination of BOL in rat plasma. The samples were treated by simple liquid-liquid extraction with ethyl acetate and separated on an UPLC BEH C18 column (2.1 mm × 50 mm) using a 3-min gradient elution scheme, which consists of water (0.1% v/v, formic acid) and methanol (0.1%, v/v, formic acid) to achieve the separation of BOL and sinomenine (IS) with high selectivity. The electrospray ionization source was used in positive ion mode; the multiple reaction monitoring quantified the target fragment ions m/z 629.6 → 569.5 for BOL and m/z 330.5 → 207.3 for IS. This work was evaluated with regards to the specificity, extraction recovery, matrix effect, linearity, accuracy, precision, stability, and dilution integrity. This approach was used to examine the pharmacokinetics of BOL in rats after oral (0.3 mg/kg) and intravenous (0.15 mg/kg) administration. BOL presented fast excretion and very low oral bioavailability.

Supporting Information