Lactobacilus Delbrueckii subsp. Bulgaricus Modulates the Secretion of Th1/Th2 and Treg Cell-Related Cytokines by PBMCs from Patients with Atopic Dermatitis

Abdolkarim Sheikhi; Hojjat Giti; Mohammad Reza Heibor; Abdollah Jafarzadeh; Mansour Shakerian; Narges Baharifar; Fereidon Niruzad; Ali Sadeghi Moghaddam; Parviz Kokhaei; Mohammad Baghaeifar

doi:10.1055/s-0043-117612

Drug Research, Table of Contents

Drug Res (Stuttg) 2017; 67(12): 724-729
DOI: 10.1055/s-0043-117612

Original Article

Lactobacilus Delbrueckii subsp. Bulgaricus Modulates the Secretion of Th1/Th2 and Treg Cell-Related Cytokines by PBMCs from Patients with Atopic Dermatitis

Authors

Abdolkarim Sheikhi

¹Dpartment of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran

²Cellular and Molecular Immunology Research laboratory, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Hojjat Giti

³Tehran Chemie Pharmaceutical Company, Tehran, Iran
Mohammad Reza Heibor

¹Dpartment of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Abdollah Jafarzadeh

⁴Department of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran
Mansour Shakerian

⁵Pegah Khuzestan Pasturized Milk co., Dezful, Iran
Narges Baharifar

¹Dpartment of Immunology, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
Fereidon Niruzad

⁶Department of Pediatric Medicine, Ganjavian Hospital, Dezful University of Medical Sciences, Dezful, Iran
Ali Sadeghi Moghaddam

⁷Faculty of Nursing and Midwifery, Dezful University of Medical Sciences, Dezful, Iran
Parviz Kokhaei

⁸Cancer Research Center, Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Mohammad Baghaeifar

⁵Pegah Khuzestan Pasturized Milk co., Dezful, Iran

Abstract

Background Atopic dermatitis (AD) is an inflammatory skin disease which may be due to the imbalance between Th1-, Th2 and Treg cell-related immune responses. Evidences suggest that appropriate stimulation with probiotics may correct the skewed immune response in children with AD. The aim was to determine the effects of the yogurt culture lactobacillus Bulgaricus on the secretion of Th1/Th2/Treg type cytokines by PBMCs from children with AD.

Methods L. Bulgaricus was cultivated on MRS broth. The PBMCs from 20 children with AD were separated by Ficoll-Hypaque centrifugation and co-cultured with different concentrations of UV killed bacteria in RPMI-1640 plus 10% FCS for 48/72 h. The levels of IL-10, IL-4, IL-12 and IFN-γ were measured in supernatant of PBMCs by ELISA.

Results L. Bulgaricus significantly up-regulated the secretion of IL-10, IL-12 and IFN-γ, whereas decreased the secretion of IL-4 by PBMCs at both incubation times 48 h/72 h and both bacteria:PBMCs ratios 100:1/50:1, compared to control (p<0.05). There were no significant differences between incubation times 48 h and 72 h regarding the secretion levels of IL-12, IFN-γ and IL-4. However, the secretion of IL-10 by L. Bulgaricus-stimulated PBMCs at incubation time 72 h and in the presence of bacteria:PBMCs ratio 100:1 was significantly higher than in incubation time 48 h and in the presence of bacteria:PBMCs ratio 50:1 (P<0.000 and P<0.00, respectively).

Conclusion These data show that L. Bulgaricus may modulate the secretion of Th1-, Th2-Treg-related cytokines in AD patients. Therefore, the possible potential therapeutic of L. Bulgaricus for treatment of AD should be consider in further investigation.

Key words

atopic dermatitis - probiotics - lactobacillus bulgaricus - Th1 - Th2 - treg cytokines

Full Text

References

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