Planta Med
DOI: 10.1055/s-0043-119643
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Icaritin Improves Antibody-Induced Thrombocytopenia in a Mouse Model by Regulating T-cell Polarization

Chenghong Sun1, 2, Jian Yang1, 2, Mingzhi Wang1, 2, Lihong Pan1, 2, Jingchun Yao1, 2, Shenglan Wang1, 2, Na Guo1, 2, Chunyan Li1, 2, Guimin Zhang1, 2
  • 1State Key Laboratory of Generic Pharmaceutical Technology for Chinese Medicine, Lunan Pharmaceutical Group Corporation, Linyi, PR China
  • 2Linyi Key Laboratory for Immunopharmacology and Immunotoxicology of Natural Medicine, Lunan Pharmaceutical Group Corporation, Linyi, PR China
Further Information

Publication History

received 24 May 2017
revised 29 August 2017

accepted 08 September 2017

Publication Date:
22 September 2017 (eFirst)

Abstract

Previous studies have shown that icaritin (ICT) has significant protective effects on immune thrombocytopenia (ITP), and the present study aimed to discuss the mechanism of this protective effect from the aspect of regulating T-cell polarization by an antibody-induced ITP mice model. Mice were given rat anti-mouse CD41 antibody (MWReg30) by intraperitoneal injection for 7 d to produce ITP model. At the same time, ICT was administrated at 10 mg/kg/d orally for 9 d. Peripheral blood platelets were counted by hematology analyzer. Spleen index was also tested. Spleen T-helper cell (Th), cytotoxic T-cell (CTL), Th1, Th2, Th17, regulatory T-cell (Treg), and follicular helper T-cell (Tfh) were quantified by flow cytometry. Serum Th1/Th2/Th17 cytokines were tested by mouse Th1/Th2/Th17 cytometric bead array (CBA) kit and transforming growth factor beta (TGF-β) were analyzed by enzyme-linked immunosorbent assay (ELISA) kit. The results indicated that ICT (10 mg/kg) protected against MWReg30-induced ITP, as evidenced by increased blood platelets and decreased spleen index. In addition, the imbalance of Th/CTL in ITP mice spleen was regulated by ICT. Meanwhile, ICT inhibited Th1, Th17, and Tfh and improved Th2 and Treg in ITP mice spleen. Furthermore, the results of CBA and ELISA suggested that ICT decreased serum Th1- and Th17-related cytokines and increased Th2 cytokines, as well as promoted the release of TGF-β. These results demonstrated that the protective effect of ICT on ITP was mediated by regulating T-cell polarization.