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Drug Res (Stuttg) 2018; 68(04): 196-204
DOI: 10.1055/s-0043-119750
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

The Anti-Inflammatory Potential of Mimosa caesalpiniifolia Following Experimental Colitis: Role of COX-2 and TNF-Alpha Expression

Marcelo Jose Dias Silva
1   Sao Paulo State University, UNESP, Campus Litoral Paulista, Sao Vicente, SP, Brazil
,
Wagner Vilegas
1   Sao Paulo State University, UNESP, Campus Litoral Paulista, Sao Vicente, SP, Brazil
,
Marcelo Aparecido da Silva
2   Department of Food and Drug, Federal University of Alfenas, UNIFAL, Alfenas, MG, Brazil
,
Ana Paula Ribeiro Paiotti
3   Department of Pathology
,
Mauricio Mercaldi Pastrelo
3   Department of Pathology
,
Pedro Luiz Menin Ruiz
4   Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil
,
Carolina Foot Gomes de Moura
4   Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil
,
Celina Tizuko Fujiyama Oshima
3   Department of Pathology
,
Daniel Araki Ribeiro
4   Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil
› Author Affiliations
Further Information

Publication History

received 03 August 2017

accepted 06 September 2017

Publication Date:
09 October 2017 (online)

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Abstract

The aim of this study was to evaluate the preventive and/or protective action of Mimosa caesalpiniifolia (M. caesalpiniifolia) following experimental colitis in rats. The rats were randomized into ten groups (n=10 per group), as follows: G1 – Sham group:; G2 – TNBS group; G3, G4 –colitis and treated with hydroalcoholic extract of M. caesalpiniifolia 250 mg/kg/day after and before/after inducing colitis, respectively; G5, G6 – colitis and treated with hydroalcoholic extract of M. caesalpiniifolia at 125 mg/kg/day after and before/after inducing colitis respectively; G7,G8 – colitis and treated with ethylacetate fraction of M. caesalpiniifolia at 50 mg/kg/day after and before/after inducing colitis, respectively; G9,G10 – colitis and treated with ethylacetate fraction of M. caesalpiniifolia at 50 mg/kg/day after and before/after inducing colitis, respectively. Rats treated with hydroalcoholic extract of M. caesalpiniifolia for both doses showed lower tissue damage in the distal colon. Ethylacetate fraction was effective at the highest dose only when administrated after inducing colitis. A downregulation of COX-2 was detected to rats suffering colitis and treated with M. caesalpiniifolia at high dose. On the other hand, TNF-alpha immunoexpression decreased in groups treated with M. caesalpiniifolia at low dose after inducing colitis. In summary, our results suggest that M. caesalpiniifolia attenuated the lesions of the colon, reduced inflammation, and modulates the expression of COX-2 and TNF-α during chronic colitis induced by TNBS when using for therapeutic purposes on a dose-dependent manner.

Key words

inflammatory bowel disease - TNBS-colitis - mimosa - TNF-alpha - COX-2
 

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