Planta Med 2018; 84(03): 176-181
DOI: 10.1055/s-0043-120447
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Polyphenols from Euphorbia pekinensis Inhibit AGEs Formation In Vitro and Vessel Dilation in Larval Zebrafish In Vivo

Ik-Soo Lee
1  KM-Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
,
Seung-Hyun Jung
2  Division of Marine-Bio Research, National Marine Biodiversity Institute of Korea, Seocheon-gun, Republic of Korea
,
Jin Sook Kim
1  KM-Based Herbal Drug Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
› Author Affiliations
Further Information

Publication History

received 25 July 2017
revised 11 September 2017

accepted 21 September 2017

Publication Date:
17 November 2017 (eFirst)

Abstract

To identify active compounds in the roots of Euphorbia pekinensis for treatment of diabetic complications, an active column fraction from a 70% EtOH extract of E. pekinensis root was purified by preparative reversed-phase high-performance liquid chromatography, leading to the isolation of a new ellagic acid derivative, 3,3′-di-O-methylellagic acid 4-O-(6ʺ-O-galloyl)-β-D-galactopyranoside (1), along with three known compounds, geraniin (2), 3,3′-di-O-methylellagic acid 4-O-β-D-xylopyranoside (3), and ellagic acid 3,3′-dimethyl ether (4). The structure of the new compound was established by extensive spectroscopic studies and chemical evidence. The inhibitory effects of isolated compounds 14 on advanced glycation end-products (AGEs) formation were examined. All compounds exhibited considerable inhibition of AGEs formation and IC50 values of 0.41 – 12.33 µM, compared with those of the positive controls aminoguanidine (IC50 = 1122.34 µM) and quercetin (IC50 = 27.80 µM). In addition, the effects of 2 and 4 on the dilation of hyaloid-retinal vessels induced by high glucose (HG) in larval zebrafish were investigated; both compounds significantly reduced the HG-induced dilation of hyaloid-retinal vessels relative to the HG-treated control group.