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Planta Medica International Open 2017; 4(03): e108-e113
DOI: 10.1055/s-0043-122397
Letters
Georg Thieme Verlag KG Stuttgart · New York

Constituents of Acacia nilotica (L.) Delile with Novel Kinase Inhibitory Activity

Augustine A. Ahmadu   *
1   Department of Pharmaceutical and Medicinal Chemistry, Kaduna State University, Kaduna, Nigeria
,
Abdulkarim Agunu
2   Department of Pharmacognosy and Drug development, University of Ilorin, Ilorin, Nigeria
,
Thi-Ngoc-Dung Nguyen
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
,
Blandine Baratte
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
,
Béatrice Foll-Josselin
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
,
Sandrine Ruchaud
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
,
Benoît Serive   *
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
,
Stéphane Bach   *
3   Sorbonne Universités, UPMC Univ. Paris 06, CNRS USR3151, Protein Phosphorylation and Human Disease Laboratory, KISSf Screening Facility, Station Biologique, Roscoff, France
› Author Affiliations
Further Information

Publication History

received 07 July 2017
revised 25 September 2017

accepted 21 October 2017

Publication Date:
05 December 2017 (online)

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Abstract

Acacia nilotica (L.) Delile belongs to the genus Acacia, which includes about 1400 species in subtropical and tropical Africa including Nigeria, Senegal, Egypt, and Mozambique as well as Asia from India to Burma. This plant is traditionally used to treat several pathologies such as mouth, ear, and bone cancer. Moreover, it possesses many other biological activities (antidiarrheal, anti-inflammatory, antimicrobial, and antifungal). We report here the extraction, purification, and identification of two known compounds [ethylgallate and (+)-catechin] from the bark of the tree that were further tested for their inhibitory activities against a panel of disease-related protein kinases. Both compounds were active, and (+)-catechin showed the best activity by inhibiting nine out of fourteen protein kinases with an IC50 value in the µg/mL range. This compound gave the highest activity against CLK1 with an IC50 of 2.1 µg/mL. The ethyl acetate extract and its components, such as catechins and other polyphenols, which also had protein kinase inhibitory activity, can be exploited in the research for anticancer agents.

Key words

Acacia nilotica - Leguminosae - (+)-catechin - ethyl gallate - protein kinases

* These authors share senior authorship.


Supporting Information

    Detailed descriptions of the isolation, structural characterization, and protein kinase assays are available online at http://www. thieme-connect.de/products as Supporting Information.
  • Supporting Information
 

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