Preliminary Study on the Expression of CLLD7 and CHC1L Proteins in Oral Squamous Cell Carcinoma

Boworn Klongnoi; Bishwa Prakash Bhattarai; Rachai Juengsomjit; Ounruean Meesakul; Sopee Poomsawat; Kajohnkiart Janebodin; Siribang-on Piboonniyom Khovidhunkit

doi:10.1055/s-0043-1768468

European Journal of Dentistry, Inhaltsverzeichnis

CC BY 4.0 · Eur J Dent 2024; 18(01): 297-303
DOI: 10.1055/s-0043-1768468

Original Article

Preliminary Study on the Expression of CLLD7 and CHC1L Proteins in Oral Squamous Cell Carcinoma

Authors

Boworn Klongnoi

¹Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Bishwa Prakash Bhattarai

¹Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

²Department of Clinical Dentistry, Walailak University International College of Dentistry, Walailak University, Bangkok, Thailand
Rachai Juengsomjit

³Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Ounruean Meesakul

³Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Sopee Poomsawat

³Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Kajohnkiart Janebodin

⁴Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Siribang-on Piboonniyom Khovidhunkit

⁵Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

Abstract

Objective This study aimed to preliminarily evaluate the expression of two putative tumor suppressor proteins, including chronic lymphocytic leukemia deletion gene 7 (CLLD7) and chromosome condensation 1-like (CHC1L) proteins in oral squamous cell carcinoma (OSCC).

Materials and Methods Expression of CLLD7 and CHC1L proteins was analyzed in 19 OSCC and 12 normal oral mucosa (NOM) using immunohistochemistry. The percentage of positive cells and intensity of staining were semiquantitatively assessed and expressed with an immunoreactive score. The number of positive cells at various subcellular localizations was evaluated and presented in percentages. The immunoreactivity scores and percentages of positive cells at various localizations were compared between the normal and OSCC groups with statistical significance at p-value less than 0.05.

Results According to immunohistochemical analysis, the immunoreactivity scores for both CLLD7 and CHC1L were higher in NOM than those of OSCC. Analysis of CLLD7 localization revealed predominant nuclear staining at basal and parabasal areas in NOM, whereas more cytoplasmic staining was observed in OSCC. For CHC1L, nuclear staining was prominent in NOM. In contrast, significantly increased plasma membrane staining was detected in OSCC.

Conclusion The expression of CLLD7 and CHC1L proteins was reduced in OSCC. Alterations in the subcellular localization of these two proteins in OSCC were also demonstrated. These preliminary results suggest that CLLD7 and CHC1L are aberrantly expressed in OSCC. The precise mechanisms of these putative tumor suppressor proteins in OSCC require future studies.

Keywords

CLLD7 - CHC1L - immunohistochemistry - oral squamous cell carcinoma - tumor suppressor proteins

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