CC BY-NC-ND 4.0 · South Asian J Cancer
DOI: 10.1055/s-0043-1768688
Original Article

Estimation of Serum Leptin, Adiponectin, and Malondialdehyde Levels in Tobacco-Induced Oral Squamous Cell Carcinoma: ELISA-Based Study

1   Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
,
1   Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
,
Anjali Narwal
1   Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
,
Paramjeet S. Gill
2   Department of Microbiology, Post Graduate Institute of Medical Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
,
Anju Devi
1   Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
,
Jagveer Yadav
1   Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences, Pandit Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana, India
› Author Affiliations
Funding All authors have no relevant financial or non-financial interest to disclose.

Abstract

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Gitika Sharma

Background Tobacco, a harmful, addictive chemical is responsible for occurrence of oral cancer by triggering inflammation and lipid peroxidation. The aim of the present study is to explore if there exists any difference in serum levels of inflammatory biomarkers such as leptin, adiponectin, and lipid peroxidation marker malondialdehyde (MDA) and also its correlation with oxidative stress in cases of tobacco-induced oral squamous cell carcinoma (OSCC) as compared with tobacco users having no lesion.

Materials and Methods Enrolled participants in this study included a total of 84 subjects (all males and 28 in each group) inclusive of healthy individuals, tobacco users with no lesion, and oral cancer cases. Serum leptin, adiponectin, and MDA levels were measured via enzyme-linked immunosorbent assay method in all subjects.

Results A significant reduction in serum leptin and adiponectin levels in patients with OSCC was observed when compared with tobacco users with no lesions and healthy persons (control). For serum MDA, findings were comparable in control and tobacco consumers with no lesions groups but significantly higher in OSCC cases. The correlation between serum adiponectin, leptin, and MDA levels with body mass index (BMI) was highly significant. In addition, comparison of BMI with serum markers and histopathological grades of OSCC showed significant difference.

Conclusion These present study observations suggest that reduced adiponectin and leptin and elevated serum MDA could serve as valuable markers for both preventive and clinical intervention, and may deserve further investigation for the early diagnosis, treatment, and prognosis of OSCC.

Ethical Approval

The study was approved by the Biomedical and Health Research Ethics Committee (PGIDS/BHRC/22/05).


Informed Consent

Informed consent was taken from each participant.


Authors' Contributions

Guarantor of the integrity of the study: G.S.


Literature research: G.S., M.K.


Data acquisition: G.S. and J.Y.


Diagnostics and management of patient: M.K., A.N., and A.D.


Manuscript preparation: G.S. and M.K.


Manuscript editing: G.S., M.K.


Manuscript review: G.S., M.K., P.S.G.




Publication History

Article published online:
09 June 2023

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