Drug Res (Stuttg) 2018; 68(10): 567-575
DOI: 10.1055/s-0044-102094
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Hepatoprotective Potential of Elsholtzia densa Against Acute and Chronic Models of Liver Damage in Wistar Rats

Ovais Ahmad Zargar
1   Department of Biochemistry, University of Kashmir, Srinagar-India
,
Rohina Bashir
1   Department of Biochemistry, University of Kashmir, Srinagar-India
,
Showkat Ahmad Ganie
1   Department of Biochemistry, University of Kashmir, Srinagar-India
,
Akbar Masood
1   Department of Biochemistry, University of Kashmir, Srinagar-India
,
Mohammad Afzal Zargar
1   Department of Biochemistry, University of Kashmir, Srinagar-India
,
Rabia Hamid
1   Department of Biochemistry, University of Kashmir, Srinagar-India
› Author Affiliations
Further Information

Publication History

received 03 December 2017

accepted 31 January 2018

Publication Date:
07 May 2018 (online)

Abstract

The aim of the present study was to evaluate the hepatoprotective activity of methanolic extract of Elsholtzia densa against experimentally induced acute (CCl4) and chronic (paracetamol) liver injury in albino wistar rats. Activity was measured by monitoring the serum levels of ALT, ALP AST and LDH, total protein levels, bilirubin and albumin. The results of the CCl4 and paracetamol-induced liver toxicity experiments showed that the rats treated with the methanolic extract of Elsholtzia densa exhibited a significant decrease in biochemical parameters as well as the proteins, which were all elevated in the CCl4 and paracetamol group. The extract at a concentration of 300 mg/kg body wt. showed a significant decline (P≤0.05) in the levels of AST, ALT, ALP and LDH to 69.50±2.23IU/L, 60.01±2.25IU/L,46.20±2.24 IU/L and 150.21±5.68IU/L in CCl4 injected animals and 51.12±2.20 IU/L,49.15±3.25 IU/L, 44.12±2.56 IU/L and 125.15±4.45 IU/L in paracetamol-treated animals when compared to the control group. The activities of tissue antioxidants GSH, GPx, GR, GST and CAT was significantly (P≤0.05) restored in dose dependent manner in animals treated with extracts as with acute and chronic hepatotoxic models. The current study confirmed the hepatoprotective effect of methanolic extract of Elsholtzia densa against the model hepatotoxicant CCl4 and paracetamol.

Supporting Information

 
  • References

  • 1 Gutteridge JMC, Halliwell B. Invited review free radicals in disease processes: A compilation of cause and consequence. Free Radical Research Communications 1993; 19: 141-158
  • 2 Halliwell B. The antioxidant paradox. The Lancet 2000; 355: 1179-1180
  • 3 Singhal KG, Gupta GD. Hepatoprotective and antioxidant activity of methanolic extract of flowers of Nerium oleander against CCl4“induced liver injury in rats. Asian Pacific Journal of Tropical Medicine 2012; 5: 677-685
  • 4 Sagar R, Bhaiji A, Toppo FA. et al. A comprehensive review on herbal drugs for hepatoprotection of 21 st Century. International Journal of Nutrition, Pharmacology, Neurological Diseases 2014; 4: 191
  • 5 Kumar CH. et al. A review on hepatoprotective activity of medicinal plants. International Journal of Pharmaceutical Sciences and Research 2011; 2: 501
  • 6 Galigher AE, Kozloff EN. Essentials of practical microtechnique. 1971;
  • 7 Bhaargavi V, Jyotsna GSL, Tripurana R. A review on hepatoprotactive activity. International Journal of Pharmaceutical Sciences and Research 2014; 5: 690
  • 8 Chowdhery HJ, Wadhwa BM. Flora of Himachal Pradesh: Analysis. Flora of India series 1984; 2:
  • 9 Ding C. et al. Study on chemical constituent of essential oil from Tibetan medicine (Elsholtzia ianthina). Acta Botanica Boreali-Occidentalia Sinica 2004; 24: 1929-1931
  • 10 Murugan MP. et al. Phytofoods of Nubra valley, Ladakh-the cold desert. Indian Journal of Traditional Knowledge 2010; 9: 303-308
  • 11 Ghimire SK. Medicinal and aromatic plants in the Nepal Himalaya: Status, use, sale and Conservation. 1999;
  • 12 Liu A. et al. Elsholtzia: Review of traditional uses, chemistry and pharmacology. Journal of Chinese Pharmaceutical Sciences 2007; 16: 73
  • 13 Oecd . Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure. 2002. OECD Publishing;
  • 14 Chiu H-F. et al. The pharmacological and pathological studies on several hepatic protective crude drugs from Taiwan (II). The American journal of Chinese medicine 1989; 17: 17-23
  • 15 Araya H. et al. An alteration in the liver microsomal membrane of the rat following paracetamol overdose. Journal of Pharmacy and Pharmacology 1987; 39: 1047-1049
  • 16 Walters MI, Gerarde HW. An ultra micromethod for the determination of conjugated and total bilirubin in serum or plasma. Microchemical Journal 1970; 15: 231-243
  • 17 Lowry OH. et al. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 1951; 193: 265-275
  • 18 Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromcresol green. Clinica Chimica Acta 1997; 258: 21-30
  • 19 Hoyland DV, Taylor AJ. A review of the methodology of the 2-thiobarbituric acid test. Food Chemistry 1991; 40: 271-291
  • 20 Beers RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry 1952; 195: 133-140
  • 21 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95: 351-358
  • 22 Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects 1979; 582: 67-78
  • 23 Sharma N. et al. Inhibition of benzo [a] pyrene-and cyclophoshamide-induced mutagenicity by Cinnamomum cassia. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 2001; 480: 179-188
  • 24 Paris RR, Debray M. Sur les polyphenols (acides-phenols, flavonoides) des feuilles de deux Melliacees malgches: Cedrelopsis grevei Baillon et Neobeguea mahafalensis Leroy. Plantes medicinales et phytotherapie 1972
  • 25 Ajith TA, Sheena N, Janardhanan KK. Phellinus rimosus. Protects carbon tetrachloride-induced chronic hepatotoxicity in rats: Antioxidant defense mechanism. Pharmaceutical biology 2006; 44: 467-474
  • 26 Coazkun AM. et al. Antioxidant and hepatoprotective activity of vitamin E and EGb 761 in experimental endotoxemic rats. Turkish Journal of Medical Sciences 2000; 30: 427-432
  • 27 Lim H-K. et al. Hepatoprotective effects of bergenin, a major constituent of Mallotus japonicus, on carbon tetrachloride-intoxicated rats. Journal of Ethnopharmacology 2000; 72: 469-474
  • 28 Kadiiska MB. et al. Biomarkers of oxidative stress study: Are plasma antioxidants markers of CCl 4 poisoning. Free Radical Biology and Medicine 2000; 28: 838-845
  • 29 Wang X-R, Zhai W-l. Cellular and biochemical effects in the bronchoalveolar lavage fluid of the rats exposed to fenvalerate. Chinese Journal of Pharmacology and Toxicology 1988; 4: 006
  • 30 Wong LT. et al. Pathways of disposition of acetaminophen conjugates in the mouse. Toxicology letters 1981; 9: 145-151
  • 31 Dahlin DC. et al. N-acetyl-p-benzoquinone imine: A cytochrome P-450-mediated oxidation product of acetaminophen. Proceedings of the National academy of Sciences 1984; 81: 1327-1331
  • 32 Mossa A.-T.H., Swelam ES, Mohafrash SMM. Sub-chronic exposure to fipronil induced oxidative stress, biochemical and histopathological changes in the liver and kidney of male albino rats. Toxicology Reports 2015; 2: 775-784
  • 33 Raucy JL. et al. Acetaminophen activation by human liver cytochromes P450IIE1 and P450IA2. Archives of biochemistry and biophysics 1989; 271: 270-283
  • 34 Mossa A.-T.H. et al. Amelioration of prallethrin-induced oxidative stress and hepatotoxicity in rat by the administration of Origanum majorana essential oil. BioMed Research International 2013; 2013:
  • 35 Wills PJ, Asha VV. Lygodium flexuosum extract down regulates the expression of proinflammatory cytokines in CCl4 induced hepatotoxicity. Asian Pacific Journal of Tropical Medicine 2012; 5: 421-426
  • 36 Khan MR. et al. Hepatoprotective activity of Sonchus asper against carbon tetrachloride-induced injuries in male rats: A randomized controlled trial. BMC Complementary and Alternative Medicine 2012; 12: 90