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CC BY 4.0 · Eur J Dent
DOI: 10.1055/s-0044-1789602
Original Article

In vitro Evaluation of Cytotoxicity, Biocompatibility, and Osteogenic Effect of Two Calcium Silicate Sealers Compared with AH Plus Jet

Monique Aparecida de Lima Rios Pitzschk
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Carlos Eduardo da Silveira Bueno
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Carlos Eduardo Fontana
2   PUC-Campinas, Center of Life Sciences, Postgraduate Program in Health Sciences, Campinas, São Paulo, Brazil
,
Alexandre Sigrist De Martin
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Daniel Guimarães Pedro Rocha
3   Pontifical Catholic University of Campinas, Department of Endodontics, School of Life Sciences, Campinas, São Paulo, Brazil
,
Carolina Pessoa Stringheta
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Ana Grasiela da Silva Limoeiro 
4   Department of Restorative Dentistry, Dental Materials, and Endodontics, Bauru School of Dentistry, University of São, Bauru, Brazil
,
Virgilio Marcio Bastos Braga
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Caroliny Chavier Guimaraes
5   Postgraduate Program in Dentistry, Iguaçu University, Nova Iguaçu, RJ, Brazil
,
Marilia Fagury Videira Marceliano-Alves 
5   Postgraduate Program in Dentistry, Iguaçu University, Nova Iguaçu, RJ, Brazil
6   Mauricio de Nassau University Center, Dentistry Department, Rio de Janeiro, Brazil
7   Department of Dental Research Cell, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, India
,
Wayne Martins Nascimento
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
,
Thiago Sena Guimarães 
6   Mauricio de Nassau University Center, Dentistry Department, Rio de Janeiro, Brazil
,
Rina Andrea Pelegrine 
1   Faculdade São Leopoldo Mandic, Instituto de Pesquisa São Leopoldo Mandic, Campinas, São Paulo, Brazil
› Author Affiliations Funding This study was supported by grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazilian Governmental Institutions.
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Abstract

Objective The purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet.

Materials and Methods For cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days.

Statistical Analysis MTT assay was assessed using analysis of variance (ANOVA) and Tukey’s test and over time using ANOVA and Sidak’s test. For ALP analysis, the one-way ANOVA and Student’s paired t-tests were used.

Results BCS was like AH Plus Jet. After 72 hours, BCH and BCS did not differ from AH Plus Jet and showed similar behavior in terms of viability. ALP showed a difference only after 14 days. BCH had the lowest bioactivity value. AH Plus Jet and BCS were like each other.

Conclusion AHP was more viable for SAOS-2, and the biocompatibility of Calcium silicate-based sealers was acceptable. The present finding assessed the biocompatibility of Calcium silicate bioceramic sealers, such as BCS, EndoSequence BCH, and AH Plus Jet, is acceptable and they have osteogenic effect and bioactivity.

Keywords

calcium silicate - epoxy resins - osteogenesis - root canal filling materials


Publication History

Article published online:
12 March 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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