Effects of Androgens and Estrogens and Catechol and Methoxy-Estrogen Derivatives on Mitogen-Activated Protein Kinase (ERK1,2) Activity in SW-13 Human Adrenal Carcinoma Cells

J. W. Brown; C. T. Kesler; J. T. Neary; L. M. Fishman

doi:10.1055/s-2001-14937

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2001; 33(3): 127-130
DOI: 10.1055/s-2001-14937

Original Basic

Effects of Androgens and Estrogens and Catechol and Methoxy-Estrogen Derivatives on Mitogen-Activated Protein Kinase (ERK_1,2) Activity in SW-13 Human Adrenal Carcinoma Cells[1]

J. W. Brown^{1, 2} , C. T. Kesler¹ , J. T. Neary^{1, 3} , L. M. Fishman^{1, 2}

¹ Adrenal Research Laboratory (151), V.A. Medical Center, Miami, FL 33125, U.S.A.
² Department of Medicine, University of Miami School of Medicine, Miami, FL 33101, U.S.A.
³ Department of Pathology, University of Miami School of Medicine, Miami, FL 33101, U.S.A.

Abstract

We tested the effects of 17β-estradiol as well as its catechol- and methoxy-derivatives, two androgens (DHEA and testosterone), a glucocorticoid (cortisol), a mineralocorticoid (aldosterone), and progesterone on the activity of ERK_1,2, a key component of the ERK/MAPK enzyme phosphorylation cascade, in SW-13 human adrenal carcinoma cells. After a 24-hour exposure SW-13 cells incubated with 10^-5 M concentrations of 17β-estradiol, its 2-hydroxy or its 2-methoxy derivative, all had elevated ERK activities (196 %, 159 %, and 275 %, respectively) relative to control cells (p < 0.01). Incubation with testosterone resulted in 162 % of control ERK activity (p < 0.01), whereas incubation with the far weaker androgen DHEA or with cortisol, aldosterone, or progesterone had no significant effects. These findings suggest sex steroid-specific influences in the induction or activation of signal transduction pathways known to play a crucial role in cellular proliferation and differentiation.

Key words:

Adrenal Carcinoma - Steroids - MAPK - ERK

Volltext

Referenzen

References

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1 This work was supported by the U.S. Department of Veterans Affairs.

Dr. J. W. Brown

Adrenal Research (151)
V.A. Medical Center

1201 N.W. 16 St.
Miami, FL 33125
U.S.A.

Telefon: Phone:+ 1 (305) 324-3195

Fax: Fax:+ 1 (305) 324-3126

Effects of Androgens and Estrogens and Catechol and Methoxy-Estrogen Derivatives on Mitogen-Activated Protein Kinase (ERK1,2) Activity in SW-13 Human Adrenal Carcinoma Cells[1]

Effects of Androgens and Estrogens and Catechol and Methoxy-Estrogen Derivatives on Mitogen-Activated Protein Kinase (ERK_1,2) Activity in SW-13 Human Adrenal Carcinoma Cells[1]