The Use of Cryopreserved Mature and Immature Testicular Spermatozoa for Intracytoplasmic Sperm Injection: Risks and Limitations

Wolfgang Küpker; Safaa Al-Hasani; Rainer Johannisson; Jörg Sandmann; Michael Ludwig; Dieter Jocham; Klaus Diedrich

doi:10.1055/s-2002-23517

Seminars in Reproductive Medicine, Table of Contents

Semin Reprod Med 2002; 20(1): 025-036
DOI: 10.1055/s-2002-23517

The Use of Cryopreserved Mature and Immature Testicular Spermatozoa for Intracytoplasmic Sperm Injection: Risks and Limitations

Wolfgang Küpker¹ , Safaa Al-Hasani¹ , Rainer Johannisson² , Jörg Sandmann³ , Michael Ludwig¹ , Dieter Jocham³ , Klaus Diedrich¹

¹Department of Obstetrics and Gynecology, Medical University Lübeck, Lübeck, Germany
²Institute of Pathology, Medical University Lübeck, Lübeck, Germany
³Department of Urology, Medical University Lübeck, Lübeck, Germany

Abstract

ABSTRACT

Treatment of severe male subfertility has become available since the intracytoplasmic injection of a single sperm into an oocyte was successfully applied for the first time in 1992. Moreover, with the use of fresh and cryopreserved epididymal and testicular spermatozoa for this procedure, fertilization and pregnancies could be accomplished. This review addresses the development and performance of these techniques and discusses achievements and problems as well as future aspects of the feasibility of early spermatid injection. Furthermore, limitations of these procedures and concerns with regard to genetic and epigenetic risks of using immature gametes are stressed.

KEYWORD

TESE - MESA - ROSI - ELSI - cryopreservation - imprinting

Full Text

References

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