HPLC-Based Activity Profiling of Salvia miltiorrhiza for MAO A and iNOS Inhibitory Activities

Abstract

In the course of a screening of plant extracts for potential CNS and anti-inflammatory activities, a dichloromethane extract of Salvia miltiorrhiza showed a pronounced inhibitory effect on recombinant monoamine oxidase A (MAO A) and on inducible NO synthase (iNOS) induction in Raw 267.4 cells. With the aid of HPLC-based profiling techniques, activities could be linked, to a significant extent, to peaks of tanshinone-type diterpenoids, four of which were subsequently isolated. The IC₅₀ of the most active compound, 15,16-dihydrotanshinone I, on human recombinant MAO A was at 23 μM, and 2.4 μM on lipopolysaccharide-mediated iNOS induction in Raw 267.4 cells.

Key words

Salvia miltiorrhiza - Lamiaceae - diterpenoids - tanshinones - HPLC-DAD-MS - MAO A inhibition - inhibition of iNOS induction - Raw 267.4 cells

References

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