The design of novel materials and stationary phases for the selective extraction and
fast separation of analytes from plant materials is an important part in phytomics
[1]. In fact, preconcentration and purification prior to analysis is necessary owing
to the complexity of samples. Add to this, analytes are often present in low concentrations,
what means that sample extraction, purification and preconcentration are the starting
points to successful analyses.
Within this presentation we present different strategies for the synthesis and the
modification of stationary phases to produce tailored solutions for the analytical
questions. In fact, sample preparation procedures should be shortened as much as possible
to save time and consumables and to prevent degradation of target compounds. As an
example the combination of extraction and preconcentration or/and separation within
one step using selective materials will be presented. In this coherence not only multidimensional
chromatography plays a central role, but also the use of new stationary phases within
different formats such as columns, capillaries and discs. Further on focus will be
placed on the separation of analytes and on the detection. Especially the use of matrix
assisted laser desorption ionization time of flight mass spectrometry (MALDI-ToF-MS)
for the analysis of small molecules using a newly synthesized material as matrix free
system will be presented [2]. In this coherence examples of different classes of plant
ingredients will be shown. Performance of the introduced material will be compared
with different systems described in literature accenting its effectiveness and power
for screening plant systems and metabolites.
References: 1. Stecher, G., Huck, C.W., Stöggl, W.M., Bonn, G.K. (2003), TrAC, 22: 1–14. 2. Bonn,
G.; Hashir, M.A.; Stecher, G., Bakry, R., (2006), Patent pending.
