Abstract
TLC autographic assays revealed in the CH2Cl2 extract of Tinospora crispa Miers (Menispermaceae) the presence of three compounds exhibiting antioxidant and
radical scavenging properties towards β-carotene and 2,2-diphenyl-1-picrylhydrazyl
(DPPH) radical. They were isolated and identified as N-cis-feruloyltyramine (4), (N-trans-feruloyltyramine (6) and secoisolariciresinol (5). When tested in dilution assays on the reduction of the DPPH radical, these 3 compounds
proved to be more active than the synthetic antioxidant butylhydroxytoluene (BHT).
Further investigation of the CH2Cl2 extract led to the isolation of vanillin, syringin, the alkaloid N-formylnornuciferin and the diterpene derivatives borapetosides B and C. In addition,
a LC/UV/MS analysis enabled the on-line identification of borapetoside F and N-formylannonain.
Key words
Tinospora crispa
- Menispermaceae - antioxidants - free radical scavengers - secoisolariciresinol -
N-feruloyltyramine - LC/MS