Planta Medica, Table of Contents Planta Med 1996; 62(2): 122-125DOI: 10.1055/s-2006-957832 Papers © Georg Thieme Verlag Stuttgart · New YorkMode of Antibacterial Action of Totarol, a Diterpene from Podocarpus nagi Hiroyuki Haraguchi1 , Shingo Oike1 , Hisashi Muroi1 , Isao Kubo2 1Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama 729-02, Japan 2Department of Environmental Scinece, Policy and Management, University of California, Berkeley, California 94720-3112, U.S.A. Recommend Article Abstract PDF Download(opens in new window) Buy Article(opens in new window) Abstract The antimicrobial mechanism of totarol was studied using Pseudomonas aeruginosa IFO 3080. This diterpene inhibited oxygen consumption and respiratory-driven proton trans-location in whole cells, and oxidation of NADH in membrane preparation. NADH-cytochrome c reductase was inhibited by totarol while cytochrome c oxidase was not. NADH-DPIP reductase and NADH-CoQ reductase were also inhibited. The site of respiratory inhibition of totarol was thought to be near CoQ in the bacterial electron transport chain. Key words Podocarpus nagi - Podocarpaceae - totarol - antimicrobial action - respiratory inhibition - electron transport - Pseudomonas aeruginosa PDF (296 kb)
