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Planta Med 1994; 60(3): 240-243
DOI: 10.1055/s-2006-959467
Paper

© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effect of Some Triterpenoid Saponins on Glucose Transport in Tumor Cells and its Application to in vitro Cytotoxic and Antiviral Activities

Hideo Hasegawa1 , 2 , Satoshi Matsumiya1 , Masamori Uchiyama1 , Tomonori Kurokawa2 , Yoshio Inouye2 , Ryoji Kasai2 , Sadahiko Ishibashi2 , Kazuo Yamasaki2
  • 1Itto Institute of Life Science Research, Happy World Inc., Fuchu-shi, Tokyo, Japan
  • 2Institute of Pharmaceutical Sciences, School of Medicine, Hiroshima University, Minami-ku, Hiroshima, Japan
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Publication History

1993

1993

Publication Date:
04 January 2007 (online)

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Abstract

The effects of some triterpenoid saponins on glucose transport in Ehrlich ascites tumor (EAT) cells were examined by measuring 2-deoxy-D-glucose (2-DG) uptake. The correlation of the effects with those on the growth of a human T-cell line (MT-4) and the replication of human immunodeficiency virus in MT-4 cells was also studied. Chikusetsusaponin Ia isolated from rhizomes of Panax japonicus C. A. Meyer (Araliaceae) inhibited the 2-DG uptake (IC50 = 76.3 µM) in a competitive fashion with respect to 2-DG (Ki = 0.32 mM) and the growth of MT-4 cells with CC50 of 84.4 µM, whereas it did not show any significant anti-HIV-1 activity. In contrast, zingibroside R1 isolated from rhizomes of Panax zingiberensis Wu et Feng (Araliaceae) showed some anti-HIV-1 activity, which was found to be superior to that of glycyrrhizin, as well as the inhibitory effects on the 2-DG uptake by EAT cells (IC50 = 91.3 µM) and the growth of MT-4 cells (CC50 = 46.2 µM).

Key words

Triterpenoid saponin - Panax - Araliaceae - glucose transporter - GLUT1 - 2-deoxy-D-glucose (2-DG) - Ehrlich ascites tumor (EAT) cells - human T-cell line (M T-4 cells) - HIV-1 RF (HIV-1RF)