Effects of Constituents of Beli (Aegle marmelos) on Spontaneous Beating and Calcium-Paradox of Myocardial Cells1

Nobuko Kakiuchi; Lakshmi R. E. Senaratne; Sheng-Lun Huang; Xiu-Wei Yang; Masao Hattori; Upali Pilapitiya; Tsuneo Namba

doi:10.1055/s-2006-960014

Planta Med 1991; 57(1): 43-46
DOI: 10.1055/s-2006-960014

Papers

Effects of Constituents of Beli (Aegle marmelos) on Spontaneous Beating and Calcium-Paradox of Myocardial Cells¹

Nobuko Kakiuchi² , Lakshmi R. E. Senaratne² ^, ³ , Sheng-Lun Huang² , Xiu-Wei Yang² , Masao Hattori² , Upali Pilapitiya³ , Tsuneo Namba²

²Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan
³Bandaranaike Memorial Ayurvedic Research Institute, Nawinna, Maharagama, Sri Lanka

1 Studies on cardio-active crude drugs, part III. For part II, see: Namba, T., Huang, X., Shu, Y., Huang, S., Hattori, M., Kakiuchi, N., Wang, Q., Xu, G. (1989) Planta Med. 55, 501

Abstract

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Abstract

Effects of methanolic extract of root bark of Beli (Aegle marmelos Corr.), an Ayurvedic crude drug used for heart diseases, and constituents isolated from the extract on spontaneous beating of cultured mouse myocardial cells were examined. The extract at a concentration of 100 µg/ml inhibited the beating rate by approximately 50%. Among the isolated constituents, aurapten was the most potent inhibitor; the IC₅₀ of aurapten is 0.6 µg/ml, which is comparable with that of verapamil, a calcium antagonist. Addition of aurapten at concentrations higher than 1 µg/ml significantly reduced the ratio of morphologically changed myocardial cells which originated from calcium overload caused by successive treatment with calcium-free and calcium-containing solutions.

Key words

Aegle marmelos - aurapten - calcium-overload prevention

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