HPLC determinations of the aescin contents in calli and embryoids from leaf explants
of Aesculus hippocastanum L. were carried out in order to determine whether it was possible to obtain aescin-forming
proliferations in vitro. The results indicate that embryogenic calli and embryoids derived from them produce
an amount of active principle higher than that of horse-chestnut seeds. The data are
discussed in terms of the relation between tissue differentiation and secondary metabolites
biosynthesis.
Aesculus hippocastanum
- leaf explants - callus - embryoids - aescin - HPLC
