Evaluation of Plant Extracts for Antileishmanial Activity using a Mechanism-Based Radiorespirometric Microtechnique (RAM)

Maurice M. Iwu; Joan E. Jackson; John D. Tally; Daniel L. Klayman

doi:10.1055/s-2006-961508

Planta Medica, Table of Contents

Planta Med 1992; 58(5): 436-441
DOI: 10.1055/s-2006-961508

Paper

Evaluation of Plant Extracts for Antileishmanial Activity using a Mechanism-Based Radiorespirometric Microtechnique (RAM)

Maurice M. Iwu¹ ^, ² , Joan E. Jackson³ , John D. Tally³ , Daniel L. Klayman²

¹NRC Senior Research Associate; on leave of absence from the University of Nigeria, Nsukka, Nigeria
²Department of Medicinal Chemistry, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, D.C. 20307, U.S.A.
³Department of Parasitology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Washington, D.C. 20307, U.S.A.

Abstract

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Abstract

Extracts of eleven plants used in Nigerian traditional medicine have been evaluated for possible antileishmanial activity using a radiorespirometric microtest technique based on in vitro inhibition of catabolism of ¹⁴CO₂, from a battery of ¹⁴C-substrates by promastigotes. Of 13 methanol extracts tested, 5 from Gongronema latifolia, Dorstenia multiradiata, Picralima nitida. Cola attiensis, and Desmodium gangeticnm, were active at concentrations of 50 µg/ml or less against a visceral Leishmaniaisolate.

Key words

Leishmaniasis - Gongronema latifolia - Dorstenia multiradiata - Picralima nitida - Desmodium gangeticum - Cola attiensis

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