Characterization of a Suspension Culture of Tabernaemontana elegans on Growth, Nutrient Uptake, and Accumulation of Indole Alkaloids*

R. van der Heijden; C. L. Louwe; E. R. Verhey; P. A. A. Harkes; R. Verpoorte

doi:10.1055/s-2006-961912

Planta Medica, Table of Contents

Planta Med 1989; 55(2): 158-162
DOI: 10.1055/s-2006-961912

Papers

Characterization of a Suspension Culture of Tabernaemontana elegans on Growth, Nutrient Uptake, and Accumulation of Indole Alkaloids*

R. van der Heijden¹ , C. L. Louwe¹ , E. R. Verhey¹ , P. A. A. Harkes² , R. Verpoorte¹

¹Center for Bio-Pharmaceutical Sciences, Division of Pharmacognosy, State University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands
²Division of Plant Molecular Biology, Subfaculty of Biology, State University of Leiden, Nonnensteeg 3, 2311 VJ Leiden, The Netherlands

* Part 22 in the series “Pharmacognostical studies of Tabernaemontana species”. For part 21, see Ref. (1)

Abstract

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Abstract

A suspension culture of Tabernaemontana elegans was characterized on growth, nutrient uptake (carbohydrate, nitrogen, and phosphate), and on accumulation of indole alkaloids. Besides tryptamine seven indole alkaloids were isolated and identified: vobasine, vobasinol, perivine, isositsirikine, apparicine, tubotaiwine, and O-acetylvallesamine. In spite of the presence of vobasinol as major product, only trace amounts of dimeric indole alkaloids could be detected. Alkaloid formation started at the early stationary phase.

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