Abstract
Shoot tips and axillary buds of Aconitum carmichaeli Debx. cultured in Murashige and Skoog medium supplemented with 5 ppm BAP at 25 ±
1 °C under continuous light for 6 weeks formed multiple shoots with an average of
7 shoots per culture. Multiple shoot forming ability does not decrease at least for
6 generations. Subsequent culture of these shoots on MS medium supplemented with 0.5
ppm IAA at 20 ± 1 °C under continuous light for 6 weeks resulted in root formation.
The plantlets were transplanted successfully in the soil. Alkaloid contents of clonally-propagated
plants showed less variation than those of the normally grown plants.
