Effect of Sterol Inhibitors on the Incorporation of 14C-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from Artemisia annua Tissue Cultures and Plants

G. Jose Kudakasseril; Luke Lam; E. John Staba

doi:10.1055/s-2006-962706

Planta Medica, Table of Contents

Planta Med 1987; 53(3): 280-284
DOI: 10.1055/s-2006-962706

Full Papers

Effect of Sterol Inhibitors on the Incorporation of ¹⁴ C-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from Artemisia annua Tissue Cultures and Plants

G. Jose Kudakasseril¹ , Luke Lam² , E. John Staba²

¹Department of Biology, Kenyon College, Gambier, Ohio 43022, U.S.A.
²Department of Medicinal Chemistry and Pharmacognosy, University of Minnesota, Minneapolis, Minnesota 55455, U.S.A.

Abstract

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Abstract

A cell-free system from Artemisia annua L. shoot cultures and plants (shoots and roots) which is capable of incorporating ¹⁴ C-isopentenyl pyrophosphate (¹⁴ C-IPP) into artemisinin has been developed. The sterol inhibitors miconazole, AMO 1618, CCC, and MER 29 increased both the incorporation of ¹⁴ C-IPP into artemisinin by cell-free extracts and the production of artemisinin in shoot cultures of Artemisia annua.

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Effect of Sterol Inhibitors on the Incorporation of 14 C-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from Artemisia annua Tissue Cultures and Plants

Effect of Sterol Inhibitors on the Incorporation of ¹⁴ C-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from Artemisia annua Tissue Cultures and Plants