Summary
When polyclonal insulin antibodies were preincubated with either adipose tissue from
hypophysectomized rats or adipocytes from normal rats, human growth hormone failed
to stimulate glucose oxidation. Removal of insulin from adipocytes through incubation
with pyruvate at pH 7.0, followed by washing three times, also abolished subsequent
in vitro insulin-like action of hGH.
Administration of the same insulin antibody to hypophysectomized rats 30 minutes prior
to injection of hGH did not inhibit the insulin-like activity of the hGH as measured
by its ability to decrease serum glucose and non-esterified fatty acid levels.
It is concluded that the in vitro promotion of glucose oxidation by hGH requires insulin. Because of the uncertainty
of complete removal of insulin in intact animals, such a conclusion cannot be made
regarding in vivo insulin-like action of hGH.
Key-Words
hGH
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Insulin-Like Activity
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Insulin Antibody
