Summary
The effect of the glucose analogs 2-deoxyglucose (2DG), glucosamine (GLA), mannoheptulose
(MAN), and gold thioglucose (GTG) on glucose utilization in the mouse ventromedial
hypothalamus (VMH) was assessed by using an in vitro incubation system. The amount
of 14C-CO2 generated from uniformly labelled 14C-glucose was measured in VMH tissue cubes. MAN and GTG did not alter glucose oxidation
at any of the concentrations compared with controls. GLA inhibited glucose utilization
but only when the amount of GLA was 3 times that of glucose in the medium. However,
this difference was not significant. The 2DG inhibited glucose oxidation significantly
(50%; P < 0.005), but only when 2DG was used in a quantity three-fold greater than
glucose. These results imply that the VMH. a reputed central area for controlling
feeding behavior, may possess a high affinity for glucose, which is not effectively
altered by competitive inhibitors.
Key-Words
Gold Thioglucose
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2-Deoxyglucose
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Mannoheptulose
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Glucosamine
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Hypothalamic Glucose Oxidation
