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Horm Metab Res 1981; 13(12): 682-685
DOI: 10.1055/s-2007-1019375
© Georg Thieme Verlag, Stuttgart · New York

Recovery of Secretin in Acid Small Intestinal Lumen Perfusates in the Rabbit

G. Lake-Bakaar, S. Tovoli, H. Li, E. Straus, R. S. Yalow
  • Solomon A. Berson Research Laboratory, Veterans Administration Medical Center, Bronx, New York, and Department of Clinical Sciences, Albert Einstein College of Medicine at the Montefiore Hospital and Medical Center, Bronx, New York, U.S.A.
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Publication History

1981

1981

Publication Date:
14 March 2008 (online)

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Summary

Immunoreactive secretin concentrations were measured in femoral venous plama and in interstinal perfusates during acid and neutral perfusion of small intestinal segments in anesthetized rabbits. Steady-state plasma and luminal secretin concentrations were <20 pg/ml. Duodenal perfusion with with 0.1 N hydrochloric acid stimulated peak plasma immunoreactive secretin concentrations of 200-300 pg/ml within five minutes. Acid stimulated plasma concentrations remained constant during 30 minutes of perfusion. Secretin output into the acid perfusate (2000-4000 pg/min) accompanied the release into plasma. Subsequent perfusion with a neutral solution resulted in a rapid return to steady-state plasma and luminal secretin concentrations. Sequential acid perfusion of the ileum, jejunum, and duodenum stimulated similar concentrations of secretin in plasma and luminal perfusates.

Key-Words:

Secretin - Radioimmunoassay - Luminal Hormone