Dietary Intake, Serum Hormones, Muscle Mass and Strength During Strength Training in 49 - 73-Year-Old Men

Abstract

Effects of dietary intake on serum hormones, muscle cross-sectional area (CSA) and strength during strength training were studied in two groups of men: 1) strength training + nutritional counseling (n = 22, 59.1 ± 6.1 yrs), and 2) strength training (n = 23, 58.5 ± 7.1 yrs). Both groups performed strength training twice a week for 21 weeks. Counseling increased carbohydrate (p < 0.01) and fiber intake (p < 0.001) and polyunsaturated/saturated fat-ratio (p < 0.05) and decreased fat intake (p < 0.01). Muscle strength and CSA increased by 16 - 20 % and by 5.4 - 5.9 % in both groups (p < 0.001). Changes in protein content of diet correlated with the changes in the acute postexercise concentrations of total (r = 0.64, p < 0.01) and free testosterone (r = 0.54, p < 0.05) after training in the counseling group. Moreover, changes in the free testosterone responses to heavy-resistance exercise correlated with the increases in the muscle CSA (r = 0.52, p < 0.05) in the counseling group. Serum basal testosterone/sex hormone-binding globulin-ratios correlated with the body mass normalized energy (kJ/kg: r = 0.54, p < 0.001), protein (g/kg: r = 0.42, p < 0.01) and fat (g/kg: r = 0.51, p < 0.01) intake in all participants during the training. The data indicate that protein and fat intake may influence serum testosterone concentrations and that the changes in exercise-induced testosterone responses may contribute to muscle mass development during strength training.

Key words

nutrition - testosterone - exercise - elderly

References

• 1 Ahtiainen J P, Pakarinen A, Alen M, Kraemer W J, Häkkinen K. Muscle hypertrophy, hormonal adaptations and strength development during strength training in strength-trained and untrained men.  Eur J Appl Physiol. 2003;  89 555-563
• 2 Bemelmans W J, Broer J, Hulshof K F, Siero F W, May J F, Meyboom-de Jong B. Long-term effects of nutritional group education for persons at high cardiovascular risk.  Eur J Public Health. 2004;  14 240-245
• 3 Campbell W W, Trappe T A, Jozsi A C, Kruskall L J, Wolfe R R, Evans W J. Dietary protein adequacy and lower body versus whole body resistive training in older humans.  J Physiol. 2002;  15 631-642
• 4 Campbell W W, Geik R A. Nutritional considerations for the older athlete.  Nutrition. 2004;  20 603-608
• 5 Chandler R M, Byrne H K, Patterson J G, Ivy J L. Dietary supplements affect the anabolic hormones after weight-training exercise.  J Appl Physiol. 1994;  76 839-845
• 6 Dorgan J F, Judd J T, Longcope C, Brown C, Schatzkin A, Clevidence B A, Campbell W S, Nair P P, Franz C, Kahle L, Taylor P R. Effects of dietary fat and fiber on plasma and urine androgens in men: a controlled feeding study.  Am J Clin Nutr. 1996;  64 850-855
• 7 Frontera W R, Meredith C N, O'Reilly K P, Knuttgen H G, Evans W J. Strength conditioning in older men: skeletal muscle hypertrophy and improved function.  J Appl Physiol. 1988;  64 1038-1044
• 8 Hughes V A, Frontera W R, Wood M, Evans W J, Dallal G E, Roubenoff R, Fiatarone Singh M A. Longitudinal muscle strength changes in older adults: influence of muscle mass, physical activity, and health.  J Gerontol A Biol Sci Med Sci. 2001;  56 B209-B217
• 9 Häkkinen K, Kallinen M, Izquierdo M, Jokelainen K, Lassila H, Mälkiä E, Kraemer W J, Newton R U, Alen M. Changes in agonist-antagonist EMG, muscle CSA, and force during strength training in middle-aged and older people.  J Appl Physiol. 1998;  84 1341-1349
• 10 Häkkinen K, Pakarinen A, Kraemer W J, Newton R U, Alen M. Basal concentrations and acute responses of serum hormones and strength development during heavy resistance training in middle-aged and elderly men and women.  J Gerontol A Biol Sci Med Sci. 2000;  55 B95-B105
• 11 Häkkinen K, Pakarinen A, Kraemer W J, Häkkinen A, Valkeinen H, Alen M. Selective muscle hypertrophy, changes in EMG and force, and serum hormones during strength training in older women.  J Appl Physiol. 2001;  91 569-580
• 12 Häkkinen K, Kraemer W J, Pakarinen A, Triplett-McBride T, McBride J M, Häkkinen A, Alen M, McGuigan M R, Bronks R, Newton R U. Effects of heavy resistance/power training on maximal strength, muscle morphology, and hormonal response patterns in 60 - 75-year-old men and women.  Can J Appl Physiol. 2002;  27 213-231
• 13 Kamel H K. Sarcopenia and aging.  Nutr Rev. 2003;  61 157-167
• 14 Koopman R, Wagenmakers A J, Manders R J, Zorenc A H, Senden J M, Gorselink M, Keizer H A, van Loon L J. The combined ingestion of protein and free leucine with carbohydrate increases post-exercise muscle protein synthesis in vivo in male subjects.  Am J Physiol. 2004;  288 E645-E653
• 15 Kraemer W J, Volek J S, Bush J A, Putukian M, Sebastianelli W J. Hormonal responses to consecutive days of heavy-resistance exercise with or without nutritional supplementation.  J Appl Physiol. 1998;  85 1544-1555
• 16 Kraemer W J, Häkkinen K, Newton R U, Nindl B C, Volek J S, McCormick M, Gotshalk L A, Gordon S E, Fleck S J, Campbell W W, Putukian M, Evans W J. Effects of heavy-resistance training on hormonal response patterns in younger vs. older men.  J Appl Physiol. 1999;  87 982-992
• 17 Lamberts S W, van den Beld A W, van der Lely A J. The endocrinology of aging.  Science. 1997;  17 419-424
• 18 Longcope C, Feldman H A, McKinlay J B, Araujo A B. Diet and sex hormone-binding globulin.  J Clin Endocrinol Metab. 2000;  85 293-296
• 19 Meredith C N, Frontera W R, O'Reilly K P, Evans W J. Body composition in elderly men: effect of dietary modification during strength training.  J Am Ger Soc. 1992;  40 155-162
• 20 Nordic Council of Ministers .4th Edition of the Nordic Nutrition Recommendations. Nord, 2004: 013 (http://www.norden.org/pub/sk/showpub.asp?pubnr=2004:013) 2004
• 21 Rantanen T, Guralnik J M, Foley D, Masaki K, Leveille S, Curb J D, White L. Midlife hand grip strength as a predictor of old age disability.  JAMA. 1999;  281 558-560
• 22 Rantanen T, Harris T, Leveille S G, Visser M, Foley D, Masaki K, Guralnik J M. Muscle strength and body mass index as long-term predictors of mortality in initially healthy men.  J Gerontol A Biol Sci Med Sci. 2000;  55 M168-M173
• 23 Roy T A, Blackman M R, Harman S M, Tobin J D, Schrager M, Metter E J. Interrelationships of serum testosterone and free testosterone index with FFM and strength in aging men.  Am J Physiol. 2002;  283 E284-E294
• 24 Sallinen J, Pakarinen A, Ahtiainen J, Kraemer W J, Volek J S, Häkkinen K. Relationship between diet and serum anabolic hormone responses to heavy resistance exercise in strength athletes and physically active males.  Int J Sports Med. 2004;  25 627-633
• 25 Tipton K D, Rasmussen B B, Miller S L, Wolf S E, Owens-Stovall S K, Petrini B E, Wolfe R R. Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise.  Am J Physiol. 2001;  281 E197-E206
• 26 Ukkola O, Gagnon J, Rankinen T, Thompson P A, Hong Y, Leon A S, Rao D C, Skinner J S, Wilmore J H, Bouchard C. Age, body mass index, race and other determinants of steroid hormone variability: the HERITAGE Family Study.  Eur J Endocrinol. 2001;  145 1-9
• 27 Volek J S. Influence of nutrition on responses to resistance training.  Med Sci Sports Exerc. 2004;  36 689-696
• 28 Wang C, Catlin D H, Starcevic B, Heber D, Ambler C, Berman N, Lucas G, Leung A, Schramm K, Lee P W, Hull L, Swerdloff R S. Low-fat high-fiber diet decreased serum and urine androgens in men.  J Clin Endocrinol Metab. 2005;  90 3550-3559