Planta Med 1983; 49(10): 115-119
DOI: 10.1055/s-2007-969827
Research Articles

© Hippokrates Verlag Stuttgart

Production of Antineoplastic Agents by Plant Tissue Cultures

I. Induction of Callus Tissues and Detection of the Agents in Cultured CellsM. Misawa1 , M. Hayashi2 , S. Takayama1
  • 1Research Laboratories for Biological Sciences, Kyowa Hakko Kogyo Co. Ltd., Ibaraki-ken, Japan
  • 2Tokyo Research Laboratory, Kyowa Hakko Kogyo Co. Ltd., Tokyo, Japan
Further Information

Publication History



Publication Date:
26 March 2007 (online)


A variety of callus tissues were induced from antineoplastic agent-producing plants such as Putterlickia verrucosa, Cephalotaxus harringtonia and Tripterygium wilfordii.

Environmental conditions to optimize cultivation of the callus tissues and the suspension cultures were examined. The cell lines tested were recognized to produce cytotoxic substances except Heliotropium indicum. Among them, T. wilfordii cultured cells produced a higher level of tripdiolide than the mother plant. Other products were preliminarily identified as expected antineoplastic agents.