An HPLC method for separation and quantitative determination of biflavones in crude
leaf extracts from Ginkgo biloba L. is described. A system using a Lichrosorb®-Diol column and the ternary elution
system: hexane-chloroform-tetrahydrofuran, was suitable for separation of sciadopitysin,
ginkgetin, isoginkgetin and bilobetin. The biflavones were detected at 330 nm; their
calibration curves were established and their response factors were calculated using
acacetin as internal standard. This method was used for the quantitative determination
of the biflavones in crude leaf extracts; the seasonal variations of the biflavone
content were studied and showed a higher amount in autumn leaves.
Ginkgo biloba
- Ginkgoaceae - Biflavones - HPLC
