The enzyme glucokinase has recently been found to be largely responsible for glucose
homeostatic responses of both the liver and pancreas. The mechanism(s) of these responses
remains unknown but recent studies suggest that the intracellular localization of
glucokinase, controlled by glucokinase regulatory protein, may be important. This
protein is known to bind to and inhibit glucokinase in a phosphofructose-sensitive
manner, and we present evidence for the interaction of these proteins with F-actin.
Glucokinase regulatory protein gelled F-actin, and gelation was specifically inhibited
by glucokinase and the regulatory protein effectors fructose-1-phosphate (F1P) and
fructose-6-phosphate (F6P). These results suggest that glucokinase regulatory protein
may play a role in metabolism-sensitive glucokinase localization in vivo.
Glucokinase Regulatory Protein - Glucokinase - F-Actin - Enzyme Localization
