Planta Med 2007; 73(11): 1230-1233
DOI: 10.1055/s-2007-981588
Biochemistry and Molecular Biology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Identification of Dryopteris crassirhizoma and the Adulterant Species Based on cpDNA rbcL and Translated Amino Acid Sequences

Z. L. Zhao1 , C. H. Leng1 , Z. T. Wang2
  • 1Department of Pharmacognosy, College of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China
  • 2Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China
Further Information

Publication History

Received: April 1, 2007 Revised: July 10, 2007

Accepted: July 11, 2007

Publication Date:
23 August 2007 (eFirst)

Abstract

Dry rhizome of Dryopteris crassirhizoma Nakai (Dryopteridaceae), also known as Guan Zhong, is a traditional Chinese herbal medicine used in the treatment of viral disease. But the dry rhizomes of Woodwardia japonica (L. f.) Sm., Osmunda japonica Thunb. and Cyrtomium fortunei J. Sm. are also used as Guan Zhong in local areas. The adulterants are similar to Dryopteris crassirhizoma. It is difficult to identify the botanical origin of these herbs. In our study, sequences of the cpDNA rbcL gene were determined and analyzed for Dryopteris crassirhizoma and adulterant species, where nineteen molecular markers had been determined. Also, amino acid sequences translated from the rbcL gene were analyzed and four important molecular markers were detected. Based on cpDNA rbcL and translated amino acid sequences, Dryopteris crassirhizoma can easily be distinguished from the other three fern species.

References

Prof. Dr. Zhao Zhili

Department of Pharmacognosy

College of Traditional Chinese Medicine

Shanghai University of Traditional Chinese Medicine

Shanghai 201203

People’s Republic of China

Phone: +86-21-5132-2202

Fax: +86-21-5132-2193

Email: zhilzhao@sohu.com