Mammea siamensis (Miq.) T. Anderson (local name Sarapi), Clusiaceae, is used in Thai indigenous medicine
as a heart tonic. The n-hexane faction of M. siamensis flowers and subfractions thereof were reported to exhibit very strong cytotoxic effect
against human CCRF-CEM leukemia cells [1]. The main compounds of M. siamensis flowers are coumarins like mammea A/AC 1, mammea A/AC cyclo D 2, mammea B/AC cyclo D 3, mammea E/AC cyclo D 4, mammea E/BA cyclo D 5, mammea E/BC cyclo D 6, mammea E/BD cyclo D 7, mammea A/AA cyclo F 8 and mammea A/AC cyclo F 9 [2–4]. Pharmacognostic and chemical characterisation of the floral parts were performed
for the quality control of the plant. Analytical investigations were performed to
determine the constituents of the flowers of M. siamensis by using thin layer chromatography (TLC), high-performance liquid chromatography
(HPLC) and liquid chromatography-mass spectrometry (LC-MS) on the basis of previously
published spectral data [5]. By these investigations, it will be possible to control
and improve the quality of M. siamensis flower raw materials for medicinal use.
1
2 R=Ph
3 R=Pr-n
4
5 R=Bu-i
6 R=Pr-n
7 R=Pr-i
8 R=Bu-i
9 R=Pr-n
Acknowledgements: C.N. is thankful to Rajamangala University of Technology Thanyaburi for a Ph.D. scholarship
and Faculty of Pharmacy, Mahidol University.
References: [1] Noysang, C. et al. (2006) The 9th International Congress on Ethnopharmacology, Nanning, Guangxi, P.R. China [2] Mahidol,
C. et al. (2002) J. Nat. Prod. 65: 757–60. [3] Phuwapraisirisan, P. et al. (2001)
ACGC Chem. Res. Commun. 13: 28–32. [4] Thebtaranonth, C. et al. (1981) Phytochem.
20: 2305–6. [5] Yang, H. et al. (2006) J. Agric. Food Chem. 54: 4114–20.
