Abstract
Introduction: Serotonin (5-HT) is involved in the regulation of food intake. In anorexia nervosa
there is a disturbance in 5-HT function. The stimulation of 5-HT2 -receptors in platelets is a useful peripheral model to investigate the cascade of
signal transduction and neuronal functioning.
Methods: 25 anorexic female patients between the ages of 11 and 18 years with a mean body
mass index (BMI) of 13.9±1.3 kg/m2 participated in this study. The 21 healthy female controls revealed a mean BMI of
20.5±2.7 kg/m2 . 5-HT stimulated intracellular Ca2+ response of the platelets was obtained using the Fura-2 method at the time of admission,
during therapy and when the target BMI was reached.
Results: We found a significant (p<0.01) decrease in 5-HT-induced Δ[Ca2+ ]i at admission and a significant (p<0.05) increase of Δ[Ca2+ ]i during treatment in patients with anorexia nervosa. Anorexic patients with and without
comorbid depression had a comparable Ca2+ release. However, low and high Ca2+ responders showed a different course of Δ[Ca2+ ]i . The treatment with antidepressants led to a significant increase of Δ[Ca2+ ]i in those patients with concomitant depression.
Discussion: Since the course of Δ[Ca2+ ]i is not related to BMI or the presence of comorbid depression, we conclude that serotonergic
transmission or signaling pathways could be disturbed in patients suffering from anorexia
nervosa. One inference of this preliminary study is that administration of antidepressants
may be more effective in patients with concomitant depression.
References
2+ as messenger of 5HT2 -receptor stimulation in human blood platelets.
Nauyn-Schmiedeberg's Arch Pharmacol.
1984;
325
337-342
2a receptor binding in patients with anorexia nervosa.
J Nucl Med.
2003;
44
163-169
2A receptor: Identity with frontal cortex serotonin 5-HT2A receptor.
J Neurochem.
1994;
63
465-469
2 receptor stimulation in platelets of depressed patients.
Biol Psychiatry.
1993;
34
565-568
2 -receptors.
Br J Pharmacol.
1985;
84
545-549
3 H]lysergic acid diethylamide binding to a 5-hydroxytryptamine receptor on human platelet
membranes.
Eur J Pharmacol.
1984;
97
87-93
2C receptors to multiple actions of central serotonin systems.
Eur J Pharmacol.
2004;
488
1-9
2 receptors in the paraventricular nucleus of the hypothalamus.
Neurosci Letters.
1993;
152
103-106
2+ indicators with greatly improved fluorescence properties.
J Biol Chem.
1985;
260
3440-3450
2+ mobilization in platelets.
Eur J Pharmacol.
1995;
288
373-377
1 recognition sites.
J Receptor Res.
1988;
8
59-81
2 receptors in human platelets via a mechanism involving protein kinase C.
J Pharmacol Exp Ther.
1990;
255
305-311
1C receptors.
Br J Pharmacol.
1988;
94
137-147
2+ response is increased in the blood platelets of depressed patients.
Biol Psychiatry.
1991;
30
310-312
2 receptor binding characteristics in the individual and the putative regulation by
serotonin.
J Neural Trans (Gen Sect).
1993;
93
27-35
3 H]paroxetine binding in patients with eating disorders.
Psychiatry Res.
2003;
118
33-38
2 and 5-HT1C mRNA and receptor levels.
Dev Brain Res.
1991;
58
51-58
2 family receptors (5-HT2A , 5-HT2B , 5-HT2C ): where structure meets function.
Pharmacol Ther.
1998;
79
231-257
2+ in human platelets is enhanced by total fasting.
Biol Psychiatry.
1997;
41
618-620
2A and 5-HT2C receptors in the rat brain.
Pharmacopsychiatry.
2006;
39
1-8
1C receptor gene.
Soc Neurosci Abs.
1993;
19
632
2+ responses in untreated depressed patients and imipramine responders in remission.
Biol Psychiatry.
1999;
45
1042-1048
Correspondence
woeckel@em.uni-frankfurt.de
