Nachweis von Atemwegsviren – Wie, warum, wann und wo?

G. Rohde; C. Drosten; I. Borg; B. Hauptmeier; F. Ringshausen; G. Schultze-Werninghaus; K. Überla

doi:10.1055/s-2008-1038292

Pneumologie 2009; 63(1): 14-22
DOI: 10.1055/s-2008-1038292

Serie: Infektiologie

Nachweis von Atemwegsviren – Wie, warum, wann und wo?

Detection of Respiratory Viruses – How, When, Where and Why?G. Rohde¹ , C. Drosten² , I. Borg¹ , B. Hauptmeier¹ , F. Ringshausen¹ , G. Schultze-Werninghaus¹ , K. Überla³

¹Medizinische Klinik III – Pneumologie, Allergologie und Schlaf- und Beatmungsmedizin, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbH, Bochum
²Institut für Virologie, Universitätsklinikum Bonn
³Abteilung für Molekulare und Medizinische Virologie, Ruhr-Universität Bochum

Abstract

Zusammenfassung

Atemwegsviren sind für die überwiegende Mehrzahl aller so genannten Erkältungskrankheiten und grippalen Infekte in der kalten Jahreszeit, akuter respiratorischen Erkrankungen im Kindesalter sowie akuter Exazerbationen von Asthma bronchiale und chronisch obstruktiver Lungenerkrankungen (COPD) verantwortlich und spielen eine nicht zu vernachlässigende Rolle bei der ambulant erworbenen Pneumonie. Leider ist der Nachweis von Atemwegsviren bis heute schwierig. In dieser Übersicht werden daher die wesentlichen Untersuchungsverfahren vorgestellt und der aktuelle Stand des Wissens über die klinische Bedeutung von Atemwegsviren bei den unterschiedlichen Erkrankungen präsentiert.

Abstract

Respiratory viruses trigger the majority of common colds, acute respiratory illnesses in children during the cold season as well as acute exacerbations of asthma and chronic obstructive pulmonary disease. They also play a role in community acquired pneumonia. Unfortunately their detection is still difficult. The aim of this review is therefore to introduce the methods of detection and to present the current knowledge of the clinical role of respiratory viruses in different diseases.

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References

Literatur

1 Modrow S, Falke D, Truyen U. Labormethoden zum Nachweis von Virusinfektionen. Molekulare Virologie. 2. ed. Heidelberg-Berlin: Spektrum Akademischer Verlag GmbH 2003: 114-129
2 Marshall D J, Reisdorf E, Harms G. et al . Evaluation of a Multiplexed PCR Assay for Detection of Respiratory Viral Pathogens in a Public Health Laboratory Setting. J Clin Microbiol. 2007; 45 3875-3882
3 Borg I, Rohde G, Loeseke S. et al . Evaluation of a quantitative real-time PCR for the detection of RSV in pulmonary diseases. Eur Respir J. 2003; 21 944-951
4 Ieven M. Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections. J Clin Virol. 2007; 40 259-276
5 Stang A, Korn K, Wildner O. et al . Characterization of Virus Isolates by Particle-Associated Nucleic Acid PCR. J Clin Microbiol. 2005; 43 716-720
6 Ambrose H E, Clewley J P. Virus discovery by sequence-independent genome amplification. Rev Med Virol. 2006; 16 365-383
7 Janeway Jr C A, Travers P, Walport M. et al .D. Structure of the antibody molecule and the immunoglobulin genes. In: Janeway Jr. CA (ed). Immunobiology: the immune system in health and disease. 4. ed. London/New York: Elsevier Science Ltd/Garland Publishing 1999: 79-113
8 Xiang X, Qiu D, Chan K P. et al . Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma. J Virol Methods. 2002; 101 127-133
9 Beckham J D, Cadena A, Lin J. et al . Respiratory viral infections in patients with chronic, obstructive pulmonary disease. J Infect. 2005; 50 322-330
10 Monto A S, Ullman B M. Acute respiratory illness in an American community. The Tecumseh study. JAMA. 1974; 227 164-169
11 Macfarlane J, Holmes W, Gard P. et al . Prospective study of the incidence, aetiology and outcome of adult lower respiratory tract illness in the community. Thorax. 2001; 56 109-114
12 Meza R A, Bridges-Webb C, Sayer G P. The management of acute bronchitis in general practice: results from the Australian Morbidity and Treatment Survey, 1990 – 1991. Aust Fam Physician. 1994; 23 1550-1553
13 Rohde G, Wiethege A, Borg I. et al . Respiratory viruses in exacerbations of chronic obstructive pulmonary disease requiring hospitalization – a case-control study. Thorax. 2003; 58 37-42
14 Forster J, Ihorst G, Rieger C H. et al . Prospective population-based study of viral lower respiratory tract infections in children under 3 years of age (the PRI.DE study). Eur J Pediatr. 2004; 163 709-716
15 Hoek L van der, Sure K, Ihorst G. et al . Croup is associated with the novel coronavirus NL63. PLoS Med 2005. Aug ; 2 (8) e240
16 Yap F H, Ho P L, Lam K F. et al . Excess hospital admissions for pneumonia, chronic obstructive pulmonary disease, and heart failure during influenza seasons in Hong Kong. J Med Virol. 2004; 73 617-623
17 Sethi S. Infectious etiology of acute exacerbations of chronic bronchitis. Chest. 2000; 117 380S-385S
18 Smith C B, Golden C A, Kanner R E. et al . Association of viral and Mycoplasma pneumoniae infections with acute respiratory illness in patients with chronic obstructive pulmonary diseases. Am Rev Respir Dis. 1980; 121 225-232
19 Gump D W, Phillips C A, Forsyth B R. et al . Role of infection in chronic bronchitis. Am Rev Respir Dis. 1976; 113 465-474
20 Buscho R O, Saxtan D, Shultz P S. et al . Infections with viruses and Mycoplasma pneumoniae during exacerbations of chronic bronchitis. J Infect Dis. 1978; 137 377-383
21 Seemungal T, Harper-Owen R, Bhowmik A. et al . Respiratory Viruses, Symptoms, and Inflammatory Markers in Acute Exacerbations and Stable Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med. 2001; 164 1618-1623
22 Hutchinson A F, Ghimire A K, Thompson M A. et al . A community-based, time-matched, case-control study of respiratory viruses and exacerbations of COPD. Respir Med. 2007; 101 2472-2481
23 Rohde G, Borg I, Arinir U. et al . Relevance of human metapneumovirus in exacerbations of COPD. Respir Res. 2005; 6 : 150 doi 0.1186/1465-9921 – 6-150
24 Hamelin M E, Cote S, Laforge J. et al . Human metapneumovirus infection in adults with community-acquired pneumonia and exacerbation of chronic obstructive pulmonary disease. Clin Infect Dis. 2005; 41 498-502
25 Martinello R A, Esper F, Weibel C. et al . Human metapneumovirus and exacerbations of chronic obstructive pulmonary disease. J Infect. 2006; 53 248-254
26 Wilkinson T M, Donaldson G C, Johnston S L. et al . Respiratory Syncytial Virus, Airway Inflammation and FEV₁ Decline in Patients with COPD. Am J Respir Crit Care Med. 2006; 173 871-876
27 Matsuse T, Hayashi S, Kuwano K. et al . Latent adenoviral infection in the pathogenesis of chronic airways obstruction. Am Rev Respir Dis. 1992; 146 177-184
28 Retamales I, Elliott W M, Meshi B. et al . Amplification of inflammation in emphysema and its association with latent adenoviral infection. Am J Respir Crit Care Med. 2001; 164 469-473
29 McManus T E, Marley A M, Baxter N. et al . Acute and latent adenovirus in COPD. Respir Med. 2007; 101 2084-2090
30 Papadopoulos N G, Xepapadaki P, Mallia P. et al . Mechanisms of virus-induced asthma exacerbations: state-of-the-art. A GA(2)LEN and InterAirways document. Allergy. 200 726; 62 457-470
31 Johnston S L, Pattemore P K, Sanderson G. et al . Community study of role of viral infections in exacerbations of asthma in 9 – 11 year old children. BMJ. 1995; 310 1225-1229
32 Nicholson K G, Kent J, Ireland D C. Respiratory viruses and exacerbations of asthma in adults. BMJ. 1993; 307 982-986
33 Johnston S L, Pattemore P K, Sanderson G. et al . The relationship between upper respiratory infections and hospital admissions for asthma: A time-trend analysis. Am J Respir Crit Care Med. 1996; 154 654-660
34 Grunberg K, Timmers M C, de Klerk E P. et al . Experimental rhinovirus 16 infection causes variable airway obstruction in subjects with atopic asthma. Am J Respir Crit Care Med. 1999; 160 1375-1380
35 Papadopoulos N G, Bates P J, Bardin P G. et al . Rhinoviruses infect the lower airways. J Infect Dis. 2000; 181 1875-1884
36 Corne J M, Marshall C, Smith S. et al . Frequency, severity, and duration of rhinovirus infections in asthmatic and non-asthmatic individuals: a longitudinal cohort study. Lancet. 2002; 359 831-834
37 Wark P A, Johnston S L, Bucchieri F. et al . Asthmatic bronchial epithelial cells have a deficient innate immune response to infection with rhinovirus. J Exp Med. 2005; 201 937-947
38 de Roux A, Marcos M A, Garcia E. et al . Viral community-acquired pneumonia in nonimmunocompromised adults. Chest. 2004; 125 1343-1351
39 Angeles M M, Camps M, Pumarola T. et al . The role of viruses in the aetiology of community-acquired pneumonia in adults. Antivir Ther. 2006; 11 351-359
40 Dowell S F, Anderson L J, Gary Jr H E. et al . Respiratory syncytial virus is an important cause of community-acquired lower respiratory infection among hospitalized adults. J Infect Dis. 1996; 174 456-462
41 Ruiz M, Ewig S, Marcos M A. et al . Etiology of community-acquired pneumonia in hospitalized patients: Impact of age, comorbidity and severity. Am J Respir Crit Care Med. 1999; 160 397-405
42 Krech T, Wegmann T, Martin H. et al . Etiology of atypical pneumonias. A serological study on 1494 patients. Schweiz Med Wochenschr. 1986; 116 2-7
43 Farr B M, Kaiser D L, Harrison B D. et al . Prediction of microbial aetiology at admission to hospital for pneumonia from the presenting clinical features. British Thoracic Society Pneumonia Research Subcommittee. Thorax. 1989; 44 1031-1035
44 Dahmash N S, Chowdhury M N. Re-evaluation of pneumonia requiring admission to an intensive care unit: a prospective study. Thorax. 1994; 49 71-76
45 Bohte R, Furth R van, Broek P J van den. Aetiology of community-acquired pneumonia: a prospective study among adults requiring admission to hospital. Thorax. 1995; 50 543-547
46 Socan M, Marinic-Fiser N, Kraigher A. et al . Microbial aetiology of community-acquired pneumonia in hospitalised patients. Eur J Clin Microbiol Infect Dis. 1999; 18 777-782
47 La S B, Marrie T J, Auffray J P. et al . Mimivirus in pneumonia patients. Emerg Infect Dis. 2005; 11 449-542
48 Woo P CY, Lau S KP, Tsoi H w. et al . Clinical and Molecular Epidemiological Features of Coronavirus HKU1-Associated Community-Acquired Pneumonia. The Journal of Infectious Diseases. 20 051; 192 1898-1907
49 Cebular S, Lee S, Tolaney P. et al . Community-acquired pneumonia in immunocompromised patients. Opportunistic infections to consider in differential diagnosis. Postgrad Med. 2003; 113 65-70, 73
50 Duffau P, Moiton M P, Lafon M E. et al . A case of severe community acquired pneumonia and non-respiratory illness induced by Influenza A. Med Mal Infect. 2006; 36 473-475
51 Lee N, Rainer T H, Ip M. et al . Role of laboratory variables in differentiating SARS-coronavirus from other causes of community-acquired pneumonia within the first 72 h of hospitalization. Eur J Clin Microbiol Infect Dis. 2006; 25 765-772
52 Jennings L C, Anderson T P, Beynon K A. et al . Incidence and Characteristics of Viral Community-Acquired Pneumonia in Adults. Thorax. 2008; 63 42-48
53 Schlosser B, Rohde G, Borg I. et al . Viral and bacterial colonization in intubated patients in the ICU. Proc Am Thorac Soc. 2005; 2 A822
54 Daubin C, Parienti J J, Vincent S. et al . Epidemiology and clinical outcome of virus-positive respiratory samples in ventilated patients: a prospective cohort study. Crit Care. 20 065; 10 R142
55 Allander T, Tammi M T, Eriksson M. et al . Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci. 2005; 102 12 891-12 896
56 Khetsuriani N, Kazerouni N N, Erdman D D. et al . Prevalence of viral respiratory tract infections in children with asthma. Journal of Allergy and Clinical Immunology. 2007; 119 314-321
57 Kleines M, Scheithauer S, Rackowitz A. et al . High Prevalence of Human Bocavirus Detected in Young Children with Severe Acute Lower Respiratory Tract Disease by Use of a Standard PCR Protocol and a Novel Real-Time PCR Protocol. J Clin Microbiol. 2007; 45 1032-104
58 Kupfer B, Vehreschild J, Cornely O. et al . Severe pneumonia and human bocavirus in adult. Emerg Infect Dis. 2006; 12 1614-1616
59 Allander T, Jartti T, Gupta S. et al . Human bocavirus and acute wheezing in children. Clin Infect Dis. 2007; 44 904-910
60 Chung J Y, Han T H, Kim S W. et al . Detection of viruses identified recently in children with acute wheezing. J Med Virol. 2007; 79 1238-1243
61 Fry A M, Lu X, Chittaganpitch M. et al . Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J Infect Dis. 2007; 195 1038-1045
62 Gendrel D, Guedj R, Pons-Catalano C. et al . Human bocavirus in children with acute asthma. Clin Infect Dis. 2007; 45 404-405
63 Kim Y J, Boeckh M, Englund J A. Community respiratory virus infections in immunocompromised patients: hematopoietic stem cell and solid organ transplant recipients, and individuals with human immunodeficiency virus infection. Semin Respir Crit Care Med. 2007; 28 222-242
64 Lee J I, Chung J Y, Han T H. et al . Detection of human bocavirus in children hospitalized because of acute gastroenteritis. J Infect Dis. 2007; 196 994-997
65 Lau S K, Yip C C, Que T L. et al . Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis. 2007; 196 986-993
66 Vicente D, Cilla G, Montes M. et al . Human bocavirus, a respiratory and enteric virus. Emerg Infect Dis. 2007; 13 636-637
67 Bastien N, Brandt K, Dust K. et al . Human Bocavirus infection, Canada. Emerg Infect Dis. 2006; 12 848-850
68 Kaplan N M, Dove W, Bu-Zeid A F. et al . Human bocavirus infection among children, Jordan. Emerg Infect Dis. 2006; 12 1418-1420
69 Neske F, Blessing K, Tollmann F. et al . Real-time PCR for diagnosis of human bocavirus infections and phylogenetic analysis. J Clin Microbiol. 2007; 45 2116-2122
70 Endo R, Ishiguro N, Kikuta H. et al . Seroepidemiology of human bocavirus in Hokkaido prefecture, Japan. J Clin Microbiol. 2007; 45 3218-3223
71 Allander T, Andreasson K, Gupta S. et al . Identification of a third human polyomavirus. J Virol. 2007; 81 4130-4136
72 Bialasiewicz S, Whiley D M, Lambert S B. et al . A newly reported human polyomavirus, KI virus, is present in the respiratory tract of Australian children. J Clin Virol. 2007; 40 15-18
73 Gaynor A M, Nissen M D, Whiley D M. et al . Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog. 2007; 3 e64
74 Norja P, Ubillos I, Templeton K. et al . No evidence for an association between infections with WU and KI polyomaviruses and respiratory disease. J Clin Virol. 2007; 40 307-311
75 Englund J A. Diagnosis and epidemiology of community-acquired respiratory virus infections in the immunocompromised host. Biol Blood Marrow Transplant. 2001; 7 2S-4S
76 Couch R B, Englund J A, Whimbey E. Respiratory viral infections in immunocompetent and immunocompromised persons. Am J Med. 1997; 102 2-9
77 Barton T D, Blumberg E A. Viral pneumonias other than cytomegalovirus in transplant recipients. Clin Chest Med. 2005; 26 707-720
78 Duncan A J, Dummer J S, Paradis I L. et al . Cytomegalovirus infection and survival in lung transplant recipients. J Heart Lung Transplant. 1991; 10 638-644
79 Ettinger N A, Bailey T C, Trulock E P. et al . Cytomegalovirus infection and pneumonitis. Impact after isolated lung transplantation. Washington University Lung Transplant Group. Am Rev Respir Dis. 1993; 147 1017-123
80 Zamora M R, Davis R D, Leonard C. Management of cytomegalovirus infection in lung transplant recipients: evidence-based recommendations. Transplantation. 2005; 80 157-163
81 Humar A, Paya C, Pescovitz M D. et al . Clinical utility of cytomegalovirus viral load testing for predicting CMV disease in D+/R− solid organ transplant recipients. Am J Transplant. 2004; 4 644-649
82 Venard V, Carret A, Corsaro D. et al . Genotyping of adenoviruses isolated in an outbreak in a bone marrow transplant unit shows that diverse strains are involved. J Hosp Infect. 2000; 44 71-74
83 Ison M G. Adenovirus infections in transplant recipients. Clin Infect Dis. 20 061; 43 331-339
84 Runde V, Ross S, Trenschel R. et al . Adenoviral infection after allogeneic stem cell transplantation (SCT): report on 130 patients from a single SCT unit involved in a prospective multi center surveillance study. Bone Marrow Transplant. 2001; 28 51-57
85 Chakrabarti S, Mautner V, Osman H. et al . Adenovirus infections following allogeneic stem cell transplantation: incidence and outcome in relation to graft manipulation, immunosuppression, and immune recovery. Blood. 2002; 100 1619-1627
86 Baldwin A, Kingman H, Darville M. et al . Outcome and clinical course of 100 patients with adenovirus infection following bone marrow transplantation. Bone Marrow Transplant. 2000; 26 1333-1338
87 Shields A F, Hackman R C, Fife K H. et al . Adenovirus infections in patients undergoing bone-marrow transplantation. N Engl J Med. 1985; 312 529-533
88 Claas E C, Schilham M W, de Brouwer C S. et al . Internally controlled real-time PCR monitoring of adenovirus DNA load in serum or plasma of transplant recipients. J Clin Microbiol. 2005; 43 1738-1744
89 Kaiser L, Aubert J D, Pache J C. et al . Chronic rhinoviral infection in lung transplant recipients. Am J Respir Crit Care Med. 2006; 174 1392-1399
90 Nichols W G, Guthrie K A, Corey L. et al . Influenza infections after hematopoietic stem cell transplantation: risk factors, mortality, and the effect of antiviral therapy. Clin Infect Dis. 2004; 39 1300-1306
91 Small T N, Casson A, Malak S F. et al . Respiratory syncytial virus infection following hematopoietic stem cell transplantation. Bone Marrow Transplant. 2002; 29 321-327
92 Lee I, Barton T D. Viral respiratory tract infections in transplant patients: epidemiology, recognition and management. Drugs. 2007; 67 1411-1427
93 Kraaij M G van, Elden L J van, Loon A M van. et al . Frequent detection of respiratory viruses in adult recipients of stem cell transplants with the use of real-time polymerase chain reaction, compared with viral culture. Clin Infect Dis. 2005; 40 662-669
94 Rohde G. Therapeutic targets in respiratory viral infections. Curr Med Chem. 2007; 14 2776-2782

Jun.-Prof. PD Dr. med. Gernot Rohde

Medizinische Klinik III, Pneumologie, Allergologie, Schlaf- und Beatmungsmedizin, Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil GmbH

Bürkle-de-la-Camp-Platz 1

44789 Bochum

Email: Gernot.Rohde@ruhr-uni-bochum.de