Eur J Pediatr Surg 2008; 18(6): 415-418
DOI: 10.1055/s-2008-1038950
Original Article

© Georg Thieme Verlag KG Stuttgart · New York

Serum Transforming Growth Factor-β1 and Epidermal Growth Factor in Biliary Atresia

P. Vejchapipat1 , A. Theamboonlers2 , S. Poomsawat3 , S. Chittmittrapap1 , Y. Poovorawan2
  • 1Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • 2Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • 3Department of Oral Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Further Information

Publication History

received June 23, 2008

accepted after revision July 7, 2008

Publication Date:
14 November 2008 (online)

Abstract

Background and Aim: Biliary atresia (BA) is a serious liver disease in children. Since transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) are involved in the hepatic reparative process, our objective was to investigate whether serum TGF-β1 and serum EGF levels were associated with therapeutic outcomes in BA. Methods: Serum levels of TGF-β1 and EGF were determined with the ELISA method in 67 postoperative BA patients with a median age of 7 years and in 10 age-comparable healthy children. The BA patients were then divided into two groups depending on their therapeutic outcome: good outcome (jaundice-free) and poor outcome (persistent jaundice). Clinical data, serum TGF-β1 and serum EGF levels were compared between the two groups of BA patients. Correlation analysis of serum TGF-β1 with serum EGF was carried out. Data are expressed as mean ± SD. Results: Serum TGF-β1 levels of BA patients were higher than those of controls (86.6 ± 15.7 vs. 75.7 ± 8.8 ng/ml, p = 0.0362). However, there was no difference in serum EGF between BA patients and controls (133.1 ± 66.6 vs. 125.4 ± 88.9 pg/ml, p = 0.744). Further subgroup analysis showed that patients with good outcomes (n = 40) had higher serum TGF-β1 and serum EGF levels than patients with poor outcomes (TGF-β1: 91.2 ± 16.5 vs. 79.6 ± 11.7 ng/ml, p = 0.002; EGF: 148.5 ± 65.0 vs. 110.3 ± 63.4 pg/ml, p = 0.02). In addition, serum TGF-β1 was positively correlated with serum EGF (Pearson's r = 0.3418, p = 0.0046). Conclusion: Elevated serum TGF-β1 and serum EGF levels were associated with a good outcome in BA patients. There was a positive correlation between serum TGF-β1 and serum EGF. This suggests that the resultant TGF-β1 and EGF pathways may be involved in the pathophysiological process in postoperative BA.

References

Dr. M.D., Ph.D. Paisarn Vejchapipat

Department of Surgery
Chulalongkorn Hospital

Rama IV road, Patumwan

10330 Bangkok

Thailand

Email: pvejchap@yahoo.co.uk