Eur J Pediatr Surg 2008; 18(5): 328-333
DOI: 10.1055/s-2008-1038959
Original Article

© Georg Thieme Verlag KG Stuttgart · New York

Application of High-Intensity Focused Ultrasound for the Treatment of Vascular Anomalies: An Experimental Study in a Cockscomb Model

S. Wang1 , J. Zhang1 , C. Li1 , X. Jin1 , C. Guo1 , Z. Wang1
  • 1Department of Pediatric Oncology Surgery, The Children's Hospital of Chongqing Medical University, Chongqing, China
Further Information

Publication History

received November 26, 2007

accepted after revision July 22, 2008

Publication Date:
14 October 2008 (online)

Abstract

Objective: We investigated the efficacy and safety of high-intensity focused ultrasound (HIFU) treatment in an animal model of vascular anomalies together with the energy efficiency relationship after varying exposure times and energy levels. Materials and Methods: Hainanhui cockscombs were irradiated using a CZP therapeutic apparatus with an optimal focus over different time periods. The changes in cuticular tissue of the cockscombs and the vascular regions were analyzed by TTC dye, H & E staining, light microscopy, and Doppler ultrasound. The rectal temperatures, weight, water and food consumption of the cocks were also measured during the whole experiment. Results: H&E staining of the tissues and combs demonstrated similar histological findings, including vessel content, and thickness of the cuticular layers (ds). There were appreciable changes to the cockscombs after doses of 2.6 w × 200 s and 3.6 w × 120 s of HIFU exposure, with 100 % effectiveness and 0.00 % and 37.50 % irreversible damage, respectively. There were no differences with regard to water consumption, weight, rectal temperature and foraging levels monitored pre- and post-HIFU administration of the two doses. Conclusions: HIFU exposure is effective in destroying the vascular targeted anomaly with no skin burns, bleeding, large vessel ruptures or other complications. HIFU is feasible, noninvasive and safe for the treatment of vascular anomalies, but clinical trials are necessary.

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Dr. Shan Wang

Department of Pediatric Oncology Surgery
The Children's Hospital of Chongqing Medical University

136 Zhongshan 2nd Rd.

Chongqing 400014

China

Email: wangshan778@163.com

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