Expression analysis of the human kallikrein 7 (KLK7) in breast tumors: a new potential biomarker for prognosis of breast carcinoma

Maroulio Talieri; Eleftherios P. Diamandis; Dimitrios Gourgiotis; Kostandina Mathioudaki; Andreas Scorilas

doi:10.1160/TH03-05-0261

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2004; 91(01): 180-186
DOI: 10.1160/TH03-05-0261

Cellular Proteolysis and Oncology

Schattauer GmbH

Expression analysis of the human kallikrein 7 (KLK7) in breast tumors: a new potential biomarker for prognosis of breast carcinoma

Maroulio Talieri

¹”G. Papanicolaou” Research Center of Oncology, “Saint Savas” Hospital, Athens, Greece

,

Eleftherios P. Diamandis

²Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Ontario, Canada

,

Dimitrios Gourgiotis

³Research Laboratories, Second Department of Pediatrics, School of Medicine, University of Athens, Athens, Greece

,

Kostandina Mathioudaki

¹”G. Papanicolaou” Research Center of Oncology, “Saint Savas” Hospital, Athens, Greece

,

Andreas Scorilas

⁴Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Athens, Greece

› Author Affiliations

Abstract

Summary

Kallikreins are a subgroup of serine proteases that are involved in the post-translational processing of polypeptide precursors. Growing evidence suggests that many kallikreins are implicated in carcinogenesis. Human kallikrein gene 7 (KLK7; HSCCE) is a new member of the human kallikrein gene family. KLK7 is expressed in normal breast tissue and is up-regulated in breast cancer cells by estrogens and glucocorticoids. In the present study, expression of the KLK7 gene in 92 breast cancer tissues was analyzed by reverse transcription-PCR (RT-PCR) and direct sequencing of several samples. The results were correlated with other clinicopathological variables and patient outcome. KLK7 gene expression was significantly lower in breast cancer patients of low stage (I/II) (p = 0.011) and patients with positive progesterone receptors (p = 0.022). Survival analysis showed that breast cancer patients with KLK7 positive tumors have relatively shorter disease-free survival (DFS) and overall survival (OS) than patients with KLK7 negative tumors. These data suggest that KLK7 gene expression may be used as a marker of unfavorable prognosis for breast cancer patients.

Keywords

KLK7 - hK7 - HSCCE - breast cancer - kallikrein - KLK - prognosis - tumor markers

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References

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