Thrombosis and Haemostasis, Table of Contents Thromb Haemost 2007; 97(04): 514-526DOI: 10.1160/TH06-05-0274 Blood Coagulation, Fibrinolysis and Cellular Haemostasis Schattauer GmbH Soluble plasma-derived von Willebrand factor assembles to a haemostatically active filamentous network Authors Author Affiliations Alexej Barg 1 Institute of Physiology II – Nanolab Rainer Ossig 2 Institute of Physiological Chemistry and Pathobiochemistry Tobias Goerge 3 Department of Dermatology, University Hospital of Muenster, Muenster, Germany 4 Currently: CBR Institute for Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachussetts, USA Matthias F. Schneider 5 Biological Physics Group, University of Augsburg, Augsburg, Germany Hermann Schillers 1 Institute of Physiology II – Nanolab Hans Oberleithner 1 Institute of Physiology II – Nanolab Stefan W. Schneider 3 Department of Dermatology, University Hospital of Muenster, Muenster, Germany Recommend Article Abstract Buy Article(opens in new window) Summary The large glycoprotein vonWillebrand factor (VWF) is involved in the initial haemostatic reaction mediating the interaction between platelets and the injured vessel wall. It has been demonstrated that unusually large VWF (ULVWF) multimers after being released from endothelium are capable of developing elongated membrane-anchored strings that are hyperactive to bind platelets. In the present study we investigated whether soluble plasma-derived VWF is competent to develop similar thrombotically active multimers. We demonstrated that soluble VWF multimers isolated from human plasma self-assemble to a network of fibers immobilized on a collagen matrix and are functionally active to bind platelets. Formation of these VWF fibers depends on shear flow, concentration of solubleVWF, and a suitable binding surface. Self-assembly of soluble VWF does not require the presence of cellular membrane ligands. The network of fibers is subjected to rapid degradation by proteolytic activity of plasma ADAMTS-13.Atomic force microscopy images elucidate the nanostructure of VWF fibers and illustrate self-association and -aggregation of several filamentous multimers. Together, these results suggest that circulatingVWF can contribute to a formation of hyperactive VWF fibers on exposed subendothelial collagen during vascular injury. Keywords KeywordsULVWF multimers - platelet adhesion - atomic force microscopy - soluble VWF Full Text References References 1 Wagner DD. New links between inflammation and thrombosis. Arterioscler Thromb Vasc Biol 2005; 25: 1321-1324. 2 Blann AD. Plasma von Willebrand factor, thrombosis, and the endothelium: The first 30 years. Thromb Haemost 2006; 95: 49-55. 3 de Groot PG, Ottenhof-Rovers M, van Mourik JA. et al. 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