Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus

Hu Hu; Linjing Zhu; Zhangsen Huang; Qiushang Ji; Madhumita Chatterjee; Wei Zhang; Nailin Li

doi:10.1160/TH10-05-0308

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2010; 104(06): 1184-1192
DOI: 10.1160/TH10-05-0308

Platelets and Blood Cells

Schattauer GmbH

Platelets enhance lymphocyte adhesion and infiltration into arterial thrombus

Authors

Hu Hu

¹Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
Linjing Zhu

¹Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China

²Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
Zhangsen Huang

¹Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China
Qiushang Ji

³Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
Madhumita Chatterjee

²Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
Wei Zhang

²Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
Nailin Li

¹Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou, China

²Karolinska Institute, Department of Medicine, Clinical Pharmacology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden

Abstract

Summary

Lymphocytes are present in atherosclerotic lesion. We hypothesise that platelets may facilitate lymphocyte infiltration into the arterial wall. Reconstituted human blood or whole blood was perfused through a collagen-coated parallel-plate flow chamber at different shear rates. Adhered platelets markedly enhanced lymphocyte adhesion that increased lymphocyte deposition from 10 ± 3 cells/mm² of platelet-depleted blood to 38 ± 11 cells/mm² of platelet-containing blood at the arterial shear rate of 500 s^-1. Platelet-dependent lymphocyte adhesion was inhibited by P-selectin, CD40L, and GPIIb/IIIa-blocking agents, suggesting the involvement of multiple adhesion molecules in this heterotypic interaction. Lymphocyte deposition was more marked among T cells, and seen in both small and large cells. B and natural killer cell adhesion was, however, mainly seen in small cells. Platelet-conjugation facilitated lymphocyte adhesion, as suggested by the selective deposition of platelet-conjugated lymphocytes. In a mouse model of arterial thrombosis, FeCl₃-induced thrombus formation markedly enhanced lymphocyte adhesion and infiltration into platelet thrombi, which was abolished by GPIIb/IIIa inhibition. In conclusion, platelets support lymphocyte adhesion under arterial flow conditions, which is selective among T cells and involves multiple adhesion molecules. Our data imply that platelets may facilitate the recruitment of circulating lymphocytes at the arterial injured sites.

Keywords

Platelets - lymphocytes - platelet-lymphocyte conjugates - arterial flow - thrombosis

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Referenzen

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