Endothelial glycocalyx thickness and platelet-vessel wall interactions during atherogenesis

Sietze Reitsma; Mirjam G. A. oude Egbrink; Viviane V. Th. Heijnen; Remco T. A. Megens; Wim Engels; Hans Vink; Dick W. Slaaf; Marc A. M. J. van Zandvoort

doi:10.1160/TH11-02-0133

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2011; 106(05): 939-946
DOI: 10.1160/TH11-02-0133

Cardiovascular Biology and Cell Signalling

Schattauer GmbH

Endothelial glycocalyx thickness and platelet-vessel wall interactions during atherogenesis

Autor*innen

Sietze Reitsma

¹Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Mirjam G. A. oude Egbrink

²Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Viviane V. Th. Heijnen

²Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Remco T. A. Megens

¹Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands

³Institute for Cardiovascular Prevention, Ludwig-Maximilians University Munich, Munich, Germany
Wim Engels

¹Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Hans Vink

²Department of Physiology, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Dick W. Slaaf

¹Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands

⁴Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands
Marc A. M. J. van Zandvoort

¹Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands

⁵Institute for Molecular Cardiovascular Research (IMCAR), Universitätsklinikum Aachen, Aachen, Germany

Abstract

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Summary

The endothelial glycocalyx (EG), the luminal cover of endothelial cells, is considered to be atheroprotective. During atherogenesis, platelets adhere to the vessel wall, possibly triggered by simultaneous EG modulation. It was the objective of this study to investigate both EG thickness and platelet-vessel wall interactions during atherogenesis in the same experimental model. Intravital fluorescence microscopy was used to study platelet-vessel wall interactions in vivo in common carotid arteries and bifurcations of C57bl6/J (B6) and apolipoprotein E knock-out (ApoE-/-) mice (age 7 – 31 weeks). At the same locations, EG thickness was determined ex vivo using two-photon laser scanning microscopy. In ApoE-/- bifurcations the overall median level of adhesion was 48 platelets/mm2 (interquartile range: 16 – 80), which was significantly higher than in B6 bifurcations (0 (0 – 16), p = 0.001). This difference appeared to result from a significant age-dependent increase in ApoE-/- mice, while no such change was observed in B6 mice. At the same time, the EG in ApoE-/- bifurcations was significantly thinner than in B6 bifurcations (2.2 vs. 2.5 μm, respectively; p < 0.05). This resulted from the fact that in B6 bifurcations EG thickness increased with age (from 2.4 μm in young mice to 3.0 μm in aged ones), while in bifurcations of ApoE-/- mice this growth appeared to be absent (2.2 μm at all ages). During atherogenesis, platelet adhesion to the wall of the carotid artery bifurcation increases significantly. At the same location, EG growth with age is hampered. Therefore, glycocalyx-reinforcing strategies could possibly ameliorate atherosclerosis.

Keywords

Atherogenesis - carotid artery - endothelial glycocalyx - platelets - mice

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Referenzen

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