Circulating microparticles of glial origin and tissue factor bearing in high-grade glioma: a potential prothrombotic role

Maria Teresa Sartori; Alessandro Della Puppa; Andrea Ballin; Elena Campello; Claudia Maria Radu; Graziella Saggiorato; Domenico d’Avella; Renato Scienza; Giuseppe Cella; Paolo Simioni

doi:10.1160/TH12-12-0957

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2013; 110(02): 378-385
DOI: 10.1160/TH12-12-0957

Cellular Proteolysis and Oncology

Schattauer GmbH

Circulating microparticles of glial origin and tissue factor bearing in high-grade glioma: a potential prothrombotic role

Maria Teresa Sartori

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Alessandro Della Puppa

²Neurosurgery, Department of Neurosciences, University Hospital, Padua, Italy

,

Andrea Ballin

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Elena Campello

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Claudia Maria Radu

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Graziella Saggiorato

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Domenico d’Avella

²Neurosurgery, Department of Neurosciences, University Hospital, Padua, Italy

,

Renato Scienza

²Neurosurgery, Department of Neurosciences, University Hospital, Padua, Italy

,

Giuseppe Cella

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

,

Paolo Simioni

¹2nd Chair of Internal Medicine, Department of Cardiologic, Thoracic and Vascular Sciences, University Hospital, Padua, Italy

› Author Affiliations

Abstract

Summary

Venous thromboembolism (VTE) may complicate the clinical course of glioblastoma multiforme (GBM). Circulating microparticles (MPs) have been associated with cancer-related VTE. Sixty-one consecutive patients with GBM undergoing gross-total (41) or subtotal (20) surgical resection followed by radio-chemotherapy were prospectively evaluated. MPs numbers according to cellular origin and the procoagulant activity of annexin V positive (AV+) MPs (MP-activity) were measured before surgery and then 1 week and 1, 4, and 7 months after surgery. Glial (GFAP+) and endothelial (CD62E+) derived MPs, AV+ and tissue factor-bearing (TF+) MPs were measured using flow cytometry. Baseline levels of GFAP+/TF-, TF+/GFAP-, and GFAP+/TF+ MPs were significantly higher in GBM patients than in healthy controls, and significantly increased at each time point after surgery; at 7 months, a further significant increase over the level found a week after surgery was only seen in the subtotally resected patients. The number AV+/CD62E- MPs increased in GBM patients and correlated with MP activity. TF+/GFAP- MPs numbers were significantly higher in 11 GBM patients who developed VTE than in those who did not (p 0.04). TF+/GFAP- MPs levels above the 90th percentile (calculated in GBM patients without VTE) were associated with a higher risk of VTE (RR 4.17, 95% CI 1.57–11.03). In conclusion, the numbers of glial-derived and/or TF-bearing MPs were high in GBM patients both before and even more after the neoplasm was treated, especially in patients with subtotal resection likely according to disease progression. A contribution of TF+/GFAP- MPs to the risk of VTE is suggested.

Keywords

Glioblastoma multiforme - microparticles - tissue factor - tissue factor bearing microparticles - venous thromboembolism

Full Text

References

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