Effects of high-amount–high-intensity exercise on in vivo platelet activation: Modulation by lipid peroxidation and AGE/RAGE axis

Francesca Santilli; Natale Vazzana; Pierpaolo Iodice; Stefano Lattanzio; Rossella Liani; Rosa Grazia Bellomo; Gianfranco Lessiani; Francesca Perego; Raoul Saggini; Giovanni Davì

doi:10.1160/TH13-04-0295

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2013; 110(06): 1232-1240
DOI: 10.1160/TH13-04-0295

Platelets and Blood Cells

Schattauer GmbH

Effects of high-amount–high-intensity exercise on in vivo platelet activation: Modulation by lipid peroxidation and AGE/RAGE axis

Francesca Santilli

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Natale Vazzana

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Pierpaolo Iodice

²Department of Neuroscience and Imaging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Stefano Lattanzio

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Rossella Liani

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Rosa Grazia Bellomo

³Department of Human Movement, “G. d’Annunzio” University of Chieti

,

Gianfranco Lessiani

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Francesca Perego

⁴Internal Medicine III, Luigi Sacco Hospital, University of Milan, Milan, Italy

,

Raoul Saggini

²Department of Neuroscience and Imaging, “G. d’Annunzio” University of Chieti, Chieti, Italy

,

Giovanni Davì

¹Internal Medicine and Center of Excellence on Aging, “G. d’Annunzio” University of Chieti, Chieti, Italy

› Institutsangaben

Abstract

Summary

Physical activity is associated with cardiovascular risk reduction, but the effects of exercise on platelet activation remain controversial. We investigated the effects of regular high-amount, high intensity aerobic exercise on in vivo thromboxane (TX)-dependent platelet activation and plasma levels of platelet-derived proteins, CD40L and P-selectin, and whether platelet variables changes may be related to changes in high-density lipoprotein (HDL) and in the extent of oxidative stress and oxidative stress-related inflammation, as reflected by urinary isoprostane excretion and endogenous soluble receptor for advanced glycation end-products (esRAGE), respectively. Urinary excretion of 11-dehydro-TXB₂ and 8-iso-prostaglandin (PG)F_2α and plasma levels of P-selectin, CD40L and esRAGE were measured before and after a eight-week standardised aerobic high-amount–high-intensity training program in 22 sedentary subjects with low-to-intermediate risk. Exercise training had a clear beneficial effect on HDL cholesterol (+10%, p=0.027) and triglyceride (-27%, p=0.008) concentration. In addition, a significant (p<0.0001) decrease in urinary 11-dehydro-TXB₂ (26%), 8-iso-PGF_2α (21 %), plasma P-selectin (27%), CD40L (35%) and a 61% increase in esRAGE were observed. Multiple regression analysis revealed that urinary 8-iso-PGF_2α [beta=0.33, SEM=0.116, p=0.027] and esRAGE (beta=-0.30, SEM=31.3, p=0.046) were the only significant predictors of urinary 11-dehydro-TXB₂ excretion rate over the training period. In conclusion, regular high-amount–high-intensity exercise training has broad beneficial effects on platelet activation markers, paralleled and possibly associated with changes in the lipoprotein profile and in markers of lipid peroxidation and AGE/RAGE axis. Our findings may help explaining why a similar amount of exercise exerts significant benefits in preventing cardiovascular events.

Keywords

Aerobic exercise - platelet activation - oxidative stress - esRAGE

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Referenzen

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