Imaging the elastic modulus of human platelets during thrombininduced activation using scanning ion conductance microscopy

Johannes Rheinlaender; Sebastian Vogel; Jan Seifert; Marc Schächtele; Oliver Borst; Florian Lang; Meinrad Gawaz; Tilman E. Schäffer

doi:10.1160/TH14-05-0414

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2015; 113(02): 305-311
DOI: 10.1160/TH14-05-0414

Cellular Haemostasis and Platelets

Schattauer GmbH

Imaging the elastic modulus of human platelets during thrombininduced activation using scanning ion conductance microscopy

Autoren

Johannes Rheinlaender^†

¹Institute of Applied Physics, Eberhard Karls University Tübingen, Germany
Sebastian Vogel^†

²Department of Cardiology and Cardiovascular Diseases, Eberhard Karls University Tübingen, Germany
Jan Seifert

¹Institute of Applied Physics, Eberhard Karls University Tübingen, Germany
Marc Schächtele

¹Institute of Applied Physics, Eberhard Karls University Tübingen, Germany
Oliver Borst

²Department of Cardiology and Cardiovascular Diseases, Eberhard Karls University Tübingen, Germany
Florian Lang

³Department of Physiology, Eberhard Karls University Tübingen, Germany
Meinrad Gawaz^‡

²Department of Cardiology and Cardiovascular Diseases, Eberhard Karls University Tübingen, Germany
Tilman E. Schäffer^‡

¹Institute of Applied Physics, Eberhard Karls University Tübingen, Germany

Abstract

Summary

Platelet activation plays a critical role in haemostasis and thrombosis. It is well-known that platelets generate contractile forces during activation. However, their mechanical material properties have rarely been investigated. Here, we use scanning ion conductance microscopy (SICM) to visualise morphological and mechanical properties of live human platelets at high spatial resolution. We found that their mean elastic modulus decreases during thrombin-induced activation by about a factor of two. We observed a similar softening of platelets during cytochalasin D-induced cytoskeleton depolymerisation. However, thrombin-induced temporal and spatial modulations of the elastic modulus were substantially different from cytochalasin D-mediated changes. We thereby provide new insights into the mechanics of haemostasis and establish SICM as a novel imaging platform for the ex vivo investigation of the mechanical properties of live platelets.

Keywords

Thrombin, stiffness - scanning ion conductance microscopy - SICM - cytochalasin D

Volltext

Referenzen

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