Ovarian response and conception rate following oestrus synchronization using three protocols in Egyptian buffalo heifers

Hassan A. Hussein; Ragab H. Mohamed; Mohamed Hossam; Axel Wehrend

doi:10.15653/TPG-160214

Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere, Table of Contents

Tierarztl Prax Ausg G Grosstiere Nutztiere 2016; 44(05): 287-294
DOI: 10.15653/TPG-160214

Original Article

Schattauer GmbH

Ovarian response and conception rate following oestrus synchronization using three protocols in Egyptian buffalo heifers

Ovarielle Reaktion und Konzeptionsrate nach Östrussynchronisation mittels drei verschiedener Protokolle bei ägyptischen Büffelfärsen

Authors

Hassan A. Hussein

¹Theriogenology Department, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Ragab H. Mohamed

²Theriogenology Department, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt
Mohamed Hossam

³Ard-El-khair Buffalo Farm, Abnob, Assiut, Egypt
Axel Wehrend

⁴Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals, Justus Liebig University, Giessen, Germany

Abstract

Summary

Objective: The aim of this study was to monitor the ovarian response and conception rate following estrous synchronization using CIDR, Ovsynch and double prostaglandin F_2α protocols in Egyptian buffalo heifers. Material and methods: A total of 80 cyclic buffalo heifers were divided into four equal groups: CIDR (intravaginal progesterone releasing device, EAZI-BREED^TM CIDR^®), Ovsynch (GnRH, PGF_2α, GnRH injections), PGF (double PGF_2α doses) and control. Timed artificial insemination (TAI) was performed in all heifers. All animals were exa - mined using ultrasound and blood samples were collected for measurement of progesterone. Results: A new follicular wave occurred earlier in the Ovsynch and PGF groups than in the CIDR group (p < 0.05). The mean diameter of the ovulatory follicle was smaller in the CIDR group than in the Ovsynch and PGF groups (p < 0.05). The ovulation rate was 100% in the CIDR group, 75% in the Ovsynch group and 70% in the PGF group. In the control group a lower pregnancy rate (20%) was determined in than in the CIDR (35%), Ovsynch (40%) and PGF (35%) groups. Progesterone concentrations were numerically higher in pregnant heifers of the CIDR group but the difference was non-significantly compared to the Ovsynch, PGF and control groups (p > 0.05). Conclusion and clinical relevance: EAZIBREED^TM CIDR^®, Ovsynch-based TAI and PGF protocols were effective in synchronizing oestrus and resulted in nearly similar pregnancy rates in Egyptian buffalo heifers.

Zusammenfassung

Ziel dieser Studie war, bei ägyptischen Büffelfärsen die Reaktion der Ovarien und die Konzeptionsrate nach Östrussynchronisation mit CIDR, Ovsynch und zweimaliger PGF_2α-Gabe vergleichend zu erfassen. Material und Methoden: Insgesamt 80 zyklische Büffelfärsen wurden in vier gleich große Gruppen eingeteilt: CIDR-Gruppe (intravaginale progesteronfreisetzende Spange, EAZI-BREED^TM CIDR®), Ovsynch- Gruppe (GnRH, PGF_2α, GnRH-Injektion), PGF-Gruppe (zweimalige Gabe von PGF_2α) und Kontrollgruppe. Alle Tiere wurden terminorientiert besamt. Bei allen Tieren erfolgten eine ultrasonographische Untersuchung und eine Blutentnahme zur Progesteronbestimmung. Ergebnisse: Eine neue Follikelwelle trat in der Ovsynch- und PGFGruppe früher auf als in der CIDR-Gruppe (p < 0,05). Der mittlere Durchmesser des ovulatorischen Follikel war in der CIDR-Gruppe kleiner als in der Ovsynch- und PGF-Gruppe (p < 0,05). Die Ovulationsrate betrug in der CIDR-Gruppe 100%, in der Ovsynch-Gruppe 75% und in der PGF-Gruppe 70%. In der Kontrollgruppe ergab sich eine niedrigere Trächtigkeitsrate (20%) als in der CIDR- (35%), Ovsynch-(40%) und PGF-Gruppe (35%). Die Progesteronkonzentration lag in der CIDRGruppe höher als in der Ovsynch-, PGF- und Kontrollgruppe, doch war der Unterschied nicht signifikant (p > 0,05). Schlussfolgerung und klinische Relevanz: CIDR-, Ovsynch- und PGF-Protokolle erwiesen sich als wirksam in der Brunstsynchronisation von ägyptischen Büffel - färsen und führten zu fast gleich hohen Trächtigkeitsraten.

keywords

Reproduction - CIDR - Ovsynch - PGF_2α

Schlüsselworter

Reproduktion - CIDR - Ovsynch - PGF_2α

Full Text

References

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