The anticonvulsant effects of Ducrosia anethifolia (Boiss) essential oil are produced by its main component alpha-pinene in rats

Mahnaz Zamyad; Mehdi Abbasnejad; Saeed Esmaeili-Mahani; Ali Mostafavi; Vahid Sheibani

doi:10.1590/0004-282X20180147

Arquivos de Neuro-Psiquiatria, Table of Contents

CC BY-NC-ND 4.0 · Arq Neuropsiquiatr 2019; 77(02): 106-114
DOI: 10.1590/0004-282X20180147

Article

The anticonvulsant effects of Ducrosia anethifolia (Boiss) essential oil are produced by its main component alpha-pinene in rats

Os efeitos anticonvulsivantes do óleo essencial de Ducrosia anethifolia (Boiss) são realizados pelo seu principal componente alfa-pineno em ratos

Authors

Mahnaz Zamyad

¹Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
Mehdi Abbasnejad

¹Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
Saeed Esmaeili-Mahani

¹Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Biology, Kerman, Iran
Ali Mostafavi

²Shahid Bahonar University of Kerman, Faculty of Sciences, Department of Chemistry, Kerman, Iran
Vahid Sheibani

³Kerman University of Medical Sciences, Kerman Neuroscience Research Center (KNRC), Laboratory of Molecular Neuroscience, Kerman, Iran

Abstract

ABSTRACT

Ducrosia anethifolia has been recommended as a remedy for neurological disorders. However, the anticonvulsant effects of D. anethifolia essential oil (DAEO) and its major constituent α-pinene have not yet been clarified.

Methods: A rat model of pentylenetetrazole (PTZ)-induced convulsions was used. Oxidant and antioxidant parameters were assayed in the temporal lobe.

Results: The data showed that DAEO (50, 100 and 200 mg/kg, i.p.) and α-pinene (0.2 and 0.4 mg/kg i.p.) delayed the initiation time, and reduced the duration of myoclonic and tonic-clonic seizures following PTZ injection. The PTZ produced oxidative stress so that malondialdehyde and hydrogen peroxide levels were increased and catalase and peroxidase activity decreased. Pretreatment with DAEO and α-pinene significantly inhibited the above-mentioned enzymatic changes in PTZ-treated animals.

Conclusion: The results suggest that α-pinene, at teast in part, was responsible for the induction of the anticonvulsant and antioxidant effects of DAEO in rats.

RESUMO

A Ducrosia anethifolia tem sido recomendada como remédio para os distúrbios neurológicos. No entanto, os efeitos anticonvulsivantes do óleo essencial de Ducrosia anethifolia (DAEO) e do seu principal constituinte atfa-pineno (α-pineno) ainda não foram clarificados.

Métodos: Foi utilizado um modelo de rato de convulsões induzidas por pentilenotetrazol (PTZ). Os parâmetros oxidante e antioxidante foram ensaiados no lobo temporal do cérebro.

Resultados: Os dados mostraram que DAEO (50, 100 e 200 mg / kg, i.p.) e α-pineno (0,2 e 0,4 mg / kg i.p.) retardaram o tempo de iniciação e reduziram a duração das crises mioclçnicas e tçnico-clçnicas após a injeção de PTZ. O PTZ produziu estresse oxidativo, de modo que os níveis de malondialdeído (MDA) e de peróxido de hidrogênio aumentaram e a atividade da catalase e da peroxidase diminuiu. O pré-tratamento com DAEO e α-pineno inibiu significativamente as alterações enzimáticas mencionadas em animais tratados com PTZ.

Conclusão: O resultado sugere que α-pineno, peto menos em parte, é responsável peta indução dos efeitos anticonvulsivantes e antioxidantes da DAEO em ratos.

Keywords:

Pentytenetetrazote - seizures - oxidative stress

Palavras-chave:

Ducrosia anethifolia - α-pinene - Pentilenotetrazol - Crise - Estresse oxidativo

Full Text

References

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