Optimum Time to Detection of Bacteria and Yeast Species with BACTEC 9120 Culture System from Blood and Sterile Body FluidsSource of Support: Nil
The culture results of 4,807 blood and 383 sterile body fluid specimens received in our laboratory during a 54-month period, were analyzed to determine the time required for culture to become positive, time at which a culture could safely be considered negative, and the spectrum of isolated organisms. The specimens were processed by automated BACTEC 9120 culture system. A total of 1,677 clinically significant microorganisms were isolated. Gram positive and negative bacterial isolation rates were found to be 62.55% and 32.20%, respectively. Yeasts were recovered in 5.24%. False positivity rate was 1.5%. Clinically significant isolates recovered on day four and five were 97.81% and 99.88%, respectively. At day five, the sensitivity was 99.94% and negative predictive value 99.96%. Our data support a five-day incubation protocol for recovery of all clinically significant organisms with sensitivity reduced by 0.06%, when compared with a six-day protocol.
29 January 2020 (online)
Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India
- 1 Bloodstream infections. In: Forbes BA, Sahm DF, Weissfeld AS, editors. Bailey and Scott′s Diagnostic Microbiology. 11 th ed. Missouri: Mosby, Inc.; 2002. p. 865-83.
- 2 Durmaz G, Tercan US, Aydinli A, Kiremitci A, Kiraz N, Akgun Y. Optimum detection times for bacteria and yeast species with the BACTEC 9120 aerobic blood culture system: Evaluation for a 5-year period in a Turkish University Hospital. J Clin Microbiol 2003;41:819-21.
- 3 Reisner BS, Woods GL. Times to detection of bacteria and yeast in 9240 Blood culture bottles. J Clin Microbiol 1999;37:2024-6.
- 4 Doern GV, Brueggemann AB, Dunne WM, Jenkins SG, Halstead DC, McLaughlin JC. Four-day incubation period for blood culture bottles processed with the Difco ESP blood culture system. J Clin Microbiol 1997;35:1290-2.
- 5 Hardy DJ, Hulbert BB, Migneault PC. Time to detection of positive BacT/Alert cultures and lack of need for routine subculture of 5- to 7- day negative cultures. J Clin Microbiol 1992;30:2743-5.
- 6 Baka S, Logginidis I, Efstratiou V, Panagiotopoulou E, Kaparos G, Gerolymatos K, et al. Time to positivity of BACTEC blood culture bottles, abstr. No. 1733_441. In Abstracts of the 17 th European Congress of Clinical Microbiology and Infectious Diseases ICC, Munich, Germany, 2007.
- 7 Kara A, Kanra G, Cengiz AB, Apis M, Gür D. Pediatric blood culture: Time to positivity. Turk J Pediatr 2004;46:251-5.
- 8 Bourbeau PP, Pohlman JK. Three days of incubation may be sufficient for routine blood cultures with BacT/Alert FAN blood culture bottles. J Clin Microbiol 2001;39:2079-82.
- 9 Bourbeau PP, Foltzer M. Routine incubation of BacT/Alert FA and FN blood culture bottles for more than 3 days may not be necessary. J Clin Microbiol 2005;43:2506-9.
- 10 McGowan KL, Foster JA, Coffin SE. Outpatient pediatric blood cultures: Time to positivity. Pediatrics 2000;106:251-5.
- 11 Endimiani A, Tamborini A, Luzzaro F, Lombardi G, Toniolo A. Epidemiology of bloodstream infections and time to detection of positive blood culture: An evaluation of the automated BacT/Alert and BACTEC 9240 systems. New Microbiol 2002;25:9-16.
- 12 Johnson AS, Touchie C, Haldane DJ, Forward KR. Four-day incubation for detection of bacteremia using BACTEC 9240. Diagn Microbiol Infect Dis 2000;38:195-9.
- 13 Pauli I Jr, Shekhawat P, Kehl S, Sasidharan P. Early detection of bacteremia in the neonatal intensive care unit using the new BACTEC system. J Perinatol 1999;19:127-31.
- 14 Gray J, Brockwell M, Das I. Experience of changing between signal and BACTEC 9240 blood culture systems in a children′s hospital. J Clin Pathol 1998;51:302-5.
- 15 Kurlat I, Stoll BJ, McGowan JE. Time to positivity for detection of bateremia in neonates. J Clin Microbiol 1989;27:1068-71.
- 16 Cockerill FR 3rd, Reed GS, Hughes JG, Torgerson CA, Vetter EA, Harmsen WS, et al. Clinical comparison of BACTEC 9240 Plus Aerobic/F resin bottles and the Isolator Aerobic culture system for detection of blood stream infections. J Clin Microbiol 1997;35:1469-72.
- 17 Smith JA, Bryce EA, Ngui-Yen JH, Roberts FJ. Comparison of BACTEC 9240 and BacT/Alert blood culture system in an adult hospital. J Clin Microbiol 1995;33:1905-8.
- 18 Nolte FS, Williams JM, Jerris RC, Morello JA, Leitch CD, Matushek S, et al. Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC 9240). J Clin Microbiol 1993;31:552-7.
- 19 Weinbaum FI, Lavie S, Danek M, Sixsmith D, Heinrich GF, Mills SS. Doing it right the first time: Quality improvement and the contaminated blood culture. J Clin Microbiol 1997;35:563-5.