Effect of platelet-rich plasma concentrations on the proliferation of periodontal cells: An in vitro study

Sara Tavassoli-Hojjati; Mandana Sattari; Tayebeh Ghasemi; Rahil Ahmadi; Abbas Mashayekhi

doi:10.4103/1305-7456.195165

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(04): 469-474
DOI: 10.4103/1305-7456.195165

Original Article

Dental Investigation Society

Effect of platelet-rich plasma concentrations on the proliferation of periodontal cells: An in vitro study

Sara Tavassoli-Hojjati

¹Department of Pediatric Dentistry, Dental School, Islamic Azad University, Tehran Branch, Tehran, Iran

,

Mandana Sattari

²Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Tayebeh Ghasemi

³Department of Oral and Maxillofacial Surgery, Tehran University of Medical Sciences, Tehran, Iran

,

Rahil Ahmadi

⁴Department of Pediatric Dentistry, Dental School, Shahed University, Tehran, Iran

,

Abbas Mashayekhi

⁵Department of Dermatology, Medical School, Islamic Azad University, Markazi, Iran

› Author Affiliations

Abstract

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ABSTRACT

Objective: The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts. Materials and Methods: The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively. The plates were incubated at 37°C for 1, 2, 3, 4, and 7 days. PDL cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide proliferation assay. Statistical analysis of the data was accomplished using repeated measure ANOVA and Tukey's test. P < 0.05 was considered statistically significant. Results: The 5% PRP had the greatest effect on undifferentiated fibroblast proliferation, which was significant on the 3^rd day. There was no significant difference between 0.1% PRP and positive control during the first 3 days. The group with 50% PRP presented significantly lower proliferation, compared to other experimental and control groups. Conclusions: It may be concluded that the growth-stimulating effect of PRP is dose dependent with the best results in low concentrations.

Key words:

Avulsion - cell culture - cell proliferation - periodontal ligament cell - platelet-rich plasma

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References

REFERENCES
1 Tezel H, Atalayin C, Kayrak G. Replantation after traumatic avulsion. Eur J Dent 2013; 7: 229-32
2 Andreasen JO, Andreasen FM, Andersson L. Textbook and Color Atlas of Traumatic Injuries to the Teeth. 4th ed.. Copenhagen: Blackwell Munksgaard; 2007: 14-8 444, 450-8
3 Pohl Y, Filippi A, Kirschner H. Results after replantation of avulsed permanent teeth. I. Endodontic considerations. Dent Traumatol 2005; 21: 80-92
4 McTigue DJ. Managing traumatic injuries in the young permanent dentition. In: Pinkham JR, Casassimo PS, McTigue D, Fields HW, Nowak AJ. editors. Pediatric Dentistry, Infancy through Adolescence. 4th ed.. USA: Elsevire Saunders; 2005: 604
5 Arikan F, Becerik S, Sonmez S, Gurhan I. Effect of platelet-rich plasma on gingival and periodontal ligament fibroblasts: New in vitro growth assay. Braz J Oral Sci 2007; 23: 1432-7
6 Fréchette JP, Martineau I, Gagnon G. Platelet-rich plasmas: Growth factor content and roles in wound healing. J Dent Res 2005; 84: 434-9
7 Ntounis A, Geurs N, Vassilopoulos P, Reddy M. Clinical assessment of bone quality of human extraction sockets after conversion with growth factors. Int J Oral Maxillofac Implants 2015; 30: 196-201
8 Wang HL, Avila G. Platelet rich plasma: Myth or reality?. Eur J Dent 2007; 1: 192-4
9 Choi SH, Kim CK, Cho KS, Huh JS, Sorensen RG, Wozney JM. et al. Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP-2/ACS) on healing in 3-wall intrabony defects in dogs. J Periodontol 2002; 73: 63-72
10 Carlson NE, Roach Jr RB. Platelet-rich plasma: Clinical applications in dentistry. J Am Dent Assoc 2002; 133: 1383-6
11 Marx RE. Platelet-rich plasma: Evidence to support its use. J Oral Maxillofac Surg 2004; 62: 489-96
12 Slapnicka J, Fassmann A, Strasak L, Augustin P, Vanek J. Effects of activated and nonactivated platelet-rich plasma on proliferation of human osteoblasts in vitro . J Oral Maxillofac Surg 2008; 66: 297-301
13 Ogino Y, Ayukawa Y, Kukita T, Koyano K. The contribution of platelet-derived growth factor, transforming growth factor-beta 1, and insulin-like growth factor-I in platelet-rich plasma to the proliferation of osteoblast-like cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101: 724-9
14 Cáceres M, Hidalgo R, Sanz A, Martínez J, Riera P, Smith PC. Effect of platelet-rich plasma on cell adhesion, cell migration, and myofibroblastic differentiation in human gingival fibroblasts. J Periodontol 2008; 79: 714-20
15 Arnoczky SP, Delos D, Rodeo SA. What is platelet-rich plasma?. Oper Tech Sports Med 2011; 19: 142-8
16 Marx RE. Platelet-rich plasma (PRP): What is PRP and what is not PRP?. Implant Dent 2001; 10: 225-8
17 Graziani F, Ivanovski S, Cei S, Ducci F, Tonetti M, Gabriele M. The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clin Oral Implants Res 2006; 17: 212-9
18 Sánchez AR, Sheridan PJ, Kupp LI. Is platelet-rich plasma the perfect enhancement factor?. A current review. Int J Oral Maxillofac Implants 2003; 18: 93-103
19 Kawase T, Okuda K, Saito Y, Yoshie H. In vitro evidence that the biological effects of platelet-rich plasma on periodontal ligament cells is not mediated solely by constituent transforming-growth factor-beta or platelet-derived growth factor. J Periodontol 2005; 76: 760-7
20 Choi BH, Im CJ, Huh JY, Suh JJ, Lee SH. Effect of platelet-rich plasma on bone regeneration in autogenous bone graft. Int J Oral Maxillofac Surg 2004; 33: 56-9
21 Cenni E, Ciapetti G, Pagani S, Perut F, Giunti A, Baldini N. Effects of activated platelet concentrates on human primary cultures of fibroblasts and osteoblasts. J Periodontol 2005; 76: 323-8
22 Weibrich G, Kleis WK, Hafner G. Growth factor levels in the platelet-rich plasma produced by 2 different methods: Curasan-type PRP kit versus PCCS PRP system. Int J Oral Maxillofac Implants 2002; 17: 184-90
23 Choi BH, Zhu SJ, Kim BY, Huh JY, Lee SH, Jung JH. Effect of platelet-rich plasma (PRP) concentration on the viability and proliferation of alveolar bone cells: An in vitro study. Int J Oral Maxillofac Surg 2005; 34: 420-4
24 Setiawati EM. Natural growth factor: Platelet rich plasma stimulates proliferation of fibroblast cell culture. Indones J Trop Infect Dis 2010; 1: 102-4
25 Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998; 85: 638-46
26 Creeper F, Lichanska AM, Marshall RI, Seymour GJ, Ivanovski S. The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation. J Periodontal Res 2009; 44: 258-65
27 Han J, Meng HX, Tang JM, Li SL, Tang Y, Chen ZB. The effect of different platelet-rich plasma concentrations on proliferation and differentiation of human periodontal ligament cells in vitro . Cell Prolif 2007; 40: 241-52
28 Dugrillon A, Eichler H, Kern S, Klüter H. Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration. Int J Oral Maxillofac Surg 2002; 31: 615-9
29 Landesberg R, Roy M, Glickman RS. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 2000; 58: 297-300