99mTc-Tetrofosmin uptake correlates with the sensitivity of glioblastoma cell lines to temozolomide

George Alexiou; Xanthi Xourgia; Paraskevi Gerogianni; Evrysthenis Vartholomatos; John Kalef-Ezra; Andreas Fotopoulos; Athanasios Kyritsis

doi:10.4103/1450-1147.181155

World Journal of Nuclear Medicine, Inhaltsverzeichnis

CC BY-NC-ND 4.0 · World J Nucl Med 2017; 16(01): 45-50
DOI: 10.4103/1450-1147.181155

Original article

^99mTc-Tetrofosmin uptake correlates with the sensitivity of glioblastoma cell lines to temozolomide

Authors

George Alexiou

Neurosurgical Institute, University of Ioannina, Ioannina
Xanthi Xourgia

¹Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina
Paraskevi Gerogianni

Neurosurgical Institute, University of Ioannina, Ioannina
Evrysthenis Vartholomatos

Neurosurgical Institute, University of Ioannina, Ioannina
John Kalef-Ezra

²Department of Medical Physics, University of Ioannina, Ioannina
Andreas Fotopoulos

¹Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina
Athanasios Kyritsis

Neurosurgical Institute, University of Ioannina, Ioannina

Abstract

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^99mTc-tetrofosmin (^99mTc-TF) is a single-photon emission computed tomography tracer that has been used for brain tumor imaging. The aim of the study was to assess if ^99mTc-TF uptake by glioblastoma cells correlates with their response to temozolomide (TMZ). We investigated the correlation of TMZ antitumor effect with the ^99mTc-TF uptake in two glioblastoma cell lines. The U251MG cell line is sensitive to TMZ, whereas T98G is resistant. Viability and proliferation of the cells were examined by trypan blue exclusion assay and xCELLigence system. Cell cycle was analyzed with flow cytometry. The radioactivity in the cellular lysate was measured with a gamma scintillation counter. TMZ induced G₂/M cell cycle arrest in U251MG cells, whereas there was no effect on cell cycle in T98G cells. Lower ^99mTc-TF uptake was observed in U251MG cells that were exposed to TMZ compared to control (P = 0.0159). No significant difference in respect to ^99mTc-TF uptake was found in T98G cells when exposed to TMZ compared to control (P = 0.8). With ^99mTc-TF, it was possible to distinguish between TMZ-sensitive and resistant glioblastoma cells within 6 h of treatment initiation. Thus, ^99mTc-TF uptake may consist a novel approach to assess an early response of glioblastoma to chemotherapy and deserves further investigation.

Keywords

^99mTc-tetrofosmin - glioma - temozolomide

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